Abstract
The most successful method currently used to produce transgenic plants is based on the ability of Argobacterium tumefaciens to infect a number of higher plant species and transfer a defined DNA fragment (T-DNA) to the genome of the infected cells1. This procedure cannot be used with the agriculturally important cereals, although preliminary data have suggested2 infection of maize seedlings by A. tumefaciens strains. Purified exogenous DNA can be taken up, integrated and expressed in cells of a variety of plant species including some cereals following direct gene transfer into isolated protoplasts3–7. However, although it is possible to grow isolated cereal protoplast into unorganized tissue (calli)5,7,8, only rice protoplasts have so far been shown to regenerate mature plants9–11. Here we report an alternative approach to transformation of cereal plants which does not involve tissue culture techniques.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nester, E. W., Gordon, M. P., Amasino, R. M. & Yanofsky, M. F. A. Rev. pl. Physiol. 35, G387–413 (1984).
Graves, A. C. F. & Goldman, S. L. Pl. molec. Biol. 7, 43–50 (1986).
Paszkowski, J. et al. EMBO J. 3, 2717–2722 (1984).
Hain, R. et al. Molec. gen. Genet. 119, 161–168 (1985).
Lorz, H., Baker, B. & Schell, J. Molec. gen. Genet. 119, 178–182 (1985).
Potrykus, I., Saul, M. W., Petruska, J., Paszkowski, J. & Shillito, R. D. Molec. gen. Genet. 119, 183–188 (1985).
Fromm, M. E., Taylor, L. P. & Walbot, V. Nature 319, 791–793 (1986).
Vasil, I. K. in Cell Culture and Somatic Cell Genetics (Academic, Orlando, 1984).
Fujimura, T., Sakurai, M., Akagi, H., Negishi, T. & Hirose, A. Pl. Tissue Lett. 2, 74–75 (1985).
Yamada, Y., Zhi-Qi, Y. & Ding-Tai, T. Pl. Cell Rep. 5, 85–88 (1986).
Toriyama, Y., Hinata, K. & Sasaki, K. Theor. appl. Genet. (in the press).
De la Peña, A., Puertas, M. J. & Merino, F. Chromosoma 83, 241–248 (1981).
Puertas, M. J., De la Peña, A., Estades, B. & Merino, F. Chromosoma 89, 121–126 (1984).
Knop, W. Landwirtsch. Vers. Stn. 7, 93–107 (1885).
Ozias-Akins, P. & Vasil, I. K. in Cell Culture and Somatic Cell Genetics of Plants (Academic, London, 1985).
Reiss, B., Sprengel, R., Will, H. & Schaller, H. Gene 30, 217–223 (1984).
Schreier, P. H., Seftor, E. A., Schell, J. & Bohnert, H. J. EMBO J. 4, 25–32 (1985).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Dellaporta, S. L., Wood, J. & Hicks, J. B. Pl. molec. Biol. Rep. 1, 19–21 (1983).
Czernilofsky, A. P. et al. DNA 5, 101–103 (1986).
Merino, F. thesis. Univ. Santiago de Compostela (1986).
McQuade, H. A. & Young-Rotter, A. M. Am. J. Bot. 71, 228–238 (1984).
Heslop-Harrison, J. Ann. Bot. 30, 221–229 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de la Peña, A., Lörz, H. & Schell, J. Transgenic rye plants obtained by injecting DNA into young floral tillers. Nature 325, 274–276 (1987). https://doi.org/10.1038/325274a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/325274a0
- Springer Nature Limited
This article is cited by
-
Gene transfer to plants by electroporation: methods and applications
Molecular Biology Reports (2020)
-
Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells
Scientific Reports (2019)
-
Gene Deletion in Barley Mediated by LTR-retrotransposon BARE
Scientific Reports (2017)
-
Rice grain nutritional traits and their enhancement using relevant genes and QTLs through advanced approaches
SpringerPlus (2016)
-
Genetic analysis for rice grain quality traits in the YVB stable variant line using RAD-seq
Molecular Genetics and Genomics (2016)