Abstract
The amino-acid sequence Phe-Tyr-Met-Glu is unique to phosphorylcholine (PC)-binding antibodies1. It occurs in the first complementarity-determining region (CDR1) of the immunoglobulin heavy chains in 89% of all the anti-PC myeloma and hybridoma proteins but is not present in 490 other immunoglobulin heavy chains, 854 light chains2 or in 2,260 other unrelated proteins3. This unique tetrapeptide therefore seems to be involved in PC binding. Here we compare the effectiveness of Phe-Tyr-Met-Glu and other structurally related peptides in inhibiting the binding of PC to PC-binding proteins McPC603 and HOPC8. We also test a surface-simulation peptide4 that was constructed to mimic the combining site of McPC6035,6. Our data suggest that all these peptides inhibit the binding of PC to PC-binding proteins non-specifically and we show by computer modelling that the surface-simulation peptide does not duplicate the combining site of McPC603.
Similar content being viewed by others
References
Kabat, E. A., Wu, T. T. & Bilofsky, H. Proc. natn. Acad. Sci. U.S.A. 73, 617–619 (1976).
Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. U.S. Department of Health and Human Services, Public Health Service, National Institute of Health (1983).
Dayhoff, M. O. in Atlas of Protein Sequence and Structure (National Biomedical Research Foundation, Washington, D.C., 1984).
Kazim, A. L. & Atassi, M. Z. Biochem. J. 187, 661–666 (1980).
Segal, D. M., Padlan, E. A., Cohen, G. H., Rudikolf, S., Potter, M. & Davies, D. R. Proc. natn. Acad. Sci. U.S.A. 71, 4298–4302 (1974).
Padlan, E. A., Davies, D. R., Rudikoff, S. & Potter, M. Immunoglobulin 13, 945–949 (1976).
Wu, T. T. & Kabat, E. A. J. exp. Med. 132, 211–250 (1970).
Kabat, E. A. & Wu, T. T. Ann. N.Y. Acad. Sci. 190, 382–392 (1971).
Kocher, H. P., Berek, C., Schrier, M. H., Cosenza, H. & Jaton, J. C. Eur. J. Immun. 10, 264–267 (1980).
Clarke, S. H., Clafin, J. L. & Rudikoff, S. Proc. natn. Acad. Sci. U.S.A. 79, 3280–3284 (1982).
Gearhart, P. J., Nelson, N. D., Douglas, R. & Hood, L. E. Nature 291, 29–34 (1981).
Robinson, E. A. & Apella, E. J. biol. Chem. 254, 11418–11430 (1979).
Riesen, W. F., Braun, D. G. & Jaton, J. C. Proc. natn. Acad. Sci. U.S.A. 73, 2096–2100 (1976).
Atassi, M. A., Lee, C. L. & Pai, R. C. Biochim. biophys. Acta 427, 745–751 (1976).
Kratzin, H., Altevogt, P., Kortt, A., Ruban, E. & Hilschmann, N. Z. Physiol. Chem. 359, 1717–1745 (1978).
Kabat, E. A. Adv. Protein Chem. 32, 1–75 (1978).
Rudikoff, S. & Potter, M. Biochemistry 13, 4033–4038 (1974).
Lee, B. K. & Richards, F. M. J. molec. Biol. 55, 379–400 (1971).
Connolly, M. L. Science 221, 709–713 (1983).
Bernstein, F. C. et al. J. molec. Biol 112, 535–542 (1977).
O'Donnell, T. J. & Olson, A. J. Computer Graphics 15, 133–142 (1981).
Connolly, M. L. & Olson, A. J. Computer Chem. 9, 1–6 (1985).
Padlan, E. A. et al. in The Immune System (eds Sercarz, E. D. et al.) 7–14 (Academic, New York, 1974).
Diamond, B. & Scharff, M. D. Proc. natn. Acad. Sci. U.S.A. 81, 5841–5844 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lai, E., Rabat, E., Meienhofer, J. et al. Inhibition of phosphorylcholine binding to antibodies using synthetic peptides. Nature 325, 168–171 (1987). https://doi.org/10.1038/325168a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/325168a0
- Springer Nature Limited