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Neuronal inhibition by the peptide FMRFamide involves opening of S K+ channels

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Abstract

Neurotransmitters modulate the activity of ion channels through a variety of second messengers, including cyclic AMP1–4, cyclic GMP5,6 and the products of phosphatidylinositol breakdown7–9. Little is known about how different transmitters acting through different second-messenger systems interact within a cell to regulate single ion channels. We here describe the reciprocal actions of serotonin and the molluscan neuropeptide, FMRFamide10, on individual K+ channels in Aplysia sensory neurons. In these cells, serotonin causes prolonged all-or-none closure of a class of background conductance K+ channels (the S channels)11 through cAMP-dependent protein phosphorylation12–14. Using single-channel recording, we have found that FMRFamide produces two actions on the S channels; it increases the probablity of opening of the S channels via a cAMP-independent second-messenger system and it reverses the closures of S channels produced by serotonin or cAMP.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Center for Neurobiology and Behaviour, Department of Pharmacology, Columbia University, College of Physicians and Surgeons, 722 West 168th Street, New York, New York, 10032, USA

    F. Belardetti & S. A. Siegelbaum

  2. Howard Hughes Medical Institute, Center for Neurobiology and Behaviour, Department of Physiology, Columbia University, College of Physicians and Surgeons, 722 West 168th Street, New York, New York, 10032, USA

    E. R. Kandel

Authors
  1. F. Belardetti
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  2. E. R. Kandel
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  3. S. A. Siegelbaum
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Belardetti, F., Kandel, E. & Siegelbaum, S. Neuronal inhibition by the peptide FMRFamide involves opening of S K+ channels. Nature 325, 153–156 (1987). https://doi.org/10.1038/325153a0

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  • DOI: https://doi.org/10.1038/325153a0

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