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Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin

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Abstract

When stimulated with antigen, B cells are influenced by T cells to proliferate and differentiate into antibody-forming cells. Since it was reported1,2 that soluble factors could replace certain functions of helper T cells in the antibody response, several different kinds of lymphokines and monokines have been reported in B-cell growth and differentiation3,4. Among these, human B-cell differentiation factor (BCDF or BSF-2) has been shown to induce the final maturation of B cells into immunoglobulin-secreting cells5–8. BSF-2 was purified to homogeneity9 and its partial NH2-terminal amino-acid sequence was determined10. These studies indicated that BSF-2 is functionally and structurally unlike other known proteins. Here, we report the molecular cloning, structural analysis and functional expression of the cDNA encoding human BSF-2. The primary sequence of BSF-2 deduced from the cDNA reveals that BSF-2 is a novel interleukin consisting of 184 amino acids.

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Author notes

  1. Hisashi Harada and Tadatsugu Taniguchi: Division of Molecular Biology, Institute for Molecular and Cellular Biology, Osaka University, 1–3, Yamada-Oka, Suita, Osaka 565, Japan

  2. Akihiro Iwamatsu, Susumu Tsunasawa and Fumio Sakiyama: Division of Protein Chemistry, Institute for Protein Research, Osaka University, 1–3, Yamada-Oka, Suita, Osaka 565, Japan

  3. Hiroshi Matsui and Yoshiyuki Takahara: Central Research Laboratories, Ajinomoto Co. Inc., Totsuka-ku, Yokohama 244, Japan

Authors and Affiliations

  1. Division of Immunology, Osaka University, 1–3, Yamada-Oka, Suita, Osaka, 565, Japan

    Toshio Hirano, Kiyoshi Yasukawa, Hisashi Harada, Tetsuya Taga, Yasuo Watanabe, Tadashi Matsuda, Shin-ichiro Kashiwamura, Koichi Nakajima, Koichi Koyama, Akihiro Iwamatsu, Susumu Tsunasawa, Fumio Sakiyama, Hiroshi Matsui, Yoshiyuki Takahara, Tadatsugu Taniguchi & Tadamitsu Kishimoto

Authors
  1. Toshio Hirano
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  2. Kiyoshi Yasukawa
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  3. Hisashi Harada
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  4. Tetsuya Taga
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  5. Yasuo Watanabe
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  6. Tadashi Matsuda
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  7. Shin-ichiro Kashiwamura
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  8. Koichi Nakajima
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  9. Koichi Koyama
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  10. Akihiro Iwamatsu
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  11. Susumu Tsunasawa
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  12. Fumio Sakiyama
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  13. Hiroshi Matsui
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  14. Yoshiyuki Takahara
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  15. Tadatsugu Taniguchi
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  16. Tadamitsu Kishimoto
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Hirano, T., Yasukawa, K., Harada, H. et al. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature 324, 73–76 (1986). https://doi.org/10.1038/324073a0

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  • DOI: https://doi.org/10.1038/324073a0

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