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Extensive proliferation of mature connective-tissue type mast cells in vitro

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Abstract

There are two phenotypically distinct subpopulations of mast cells in rodents: connective tissue-type mast cells (CTMC) and mucosal mast cells (MMC). These populations differ in their location, cell size, staining characteristics, ultrastructure, mediator content and T-cell dependency1–3. Several investigators4–9 recently reported a further subclass of mast cells which arise when normal mouse haematopoietic cells are cultured with interleukin-3 (IL-3); IL-3 is an activity similar or identical to mast-cell growth factor, histamine-producing factor, or P-cell stimulating factor4–9. These cultured mast cells are in many ways similar to MMC; they stain with Alcian blue but not safranin, contain chondroitin sulphate E proteoglycan rather than heparin proteoglycan and have relatively low histamine content, as do MMC10–13. Although proliferation of MMC is known to be T-cell dependent in vivo and thought to be IL-3-dependent in vitro, the factors on which CTMC proliferation depends remain elusive. Here we show that mature CTMC purified from mouse peritoneal cells can proliferate in vitro in methylcellulose culture and maintain the appearance and function of CTMC. We also present evidence that mature CTMC cannot proliferate in the presence of pure IL-3 alone.

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Authors and Affiliations

  1. Department of Pediatrics, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, 390, Japan

    Tatsutoshi Nakahata, Toshimi Kobayashi, Akira Ishiguro, Kohichiro Tsuji, Kuniaki Naganuma, Osaaki Ando, Yoshio Yagi & Taro Akabane

  2. Department of Medicine and Physical Therapy, University of Tokyo, Tokyo, 113, Japan

    Kenji Tadokoro

Authors
  1. Tatsutoshi Nakahata
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  2. Toshimi Kobayashi
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  3. Akira Ishiguro
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  4. Kohichiro Tsuji
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  5. Kuniaki Naganuma
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  6. Osaaki Ando
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  7. Yoshio Yagi
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  8. Kenji Tadokoro
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  9. Taro Akabane
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Nakahata, T., Kobayashi, T., Ishiguro, A. et al. Extensive proliferation of mature connective-tissue type mast cells in vitro. Nature 324, 65–67 (1986). https://doi.org/10.1038/324065a0

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  • DOI: https://doi.org/10.1038/324065a0

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