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Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor

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Abstract

Cloning and sequence analysis of DNA complementary to porcine cerebral messenger RNA encoding the muscarinic acetylcholine receptor predict the complete amino-acid sequence of this protein. Expression of the complementary DNA produces functional muscarinic receptor in Xenopus oocytes. The muscarinic receptor is homologous with the β-adrenergic receptor and rhodopsin in both amino-acid sequence and suggested transmembrane topography.

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Authors and Affiliations

  1. Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Tai Kubo, Kazuhiko Fukuda, Atsushi Mikami, Akito Maeda, Hideo Takahashi, Masayoshi Mishina & Shosaku Numa

  2. Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu, 431-31, Japan

    Tatsuya Haga, Kazuko Haga & Arata Ichiyama

  3. Department of Biochemistry, Miyazaki Medical College, Miyazaki, 889-16, Japan

    Kenji Kangawa, Masayasu Kojima & Hisayuki Matsuo

  4. Pharmaceutical Institute, Keio University School of Medicine, Tokyo, 160, Japan

    Tadaaki Hirose

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  1. Tai Kubo
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Kubo, T., Fukuda, K., Mikami, A. et al. Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor. Nature 323, 411–416 (1986). https://doi.org/10.1038/323411a0

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