Abstract
Selective cloning procedures for T-cell-specific complementary DNAs have revealed the existence of a gene designated1 γ as well as the main antigen receptor α- and β-chain genes2–10. The γ-chain genes undergo rearrangement during T-cell differentiation but the patterns and complexity of such rearrangements differ markedly in mouse11–13 and human14. In mouse, a panel of cytotoxic T-lymphocyte clones exhibit the same rearrangement pattern with a γ-chain gene probe11 and a set of three γ-chain variable (V) genes have been identified in the DNA12. Clonal diversity in mouse seems to be confined to V–J (joining) regions11. In contrast, human T-cell lines exhibit diverse rearrangements14 suggestive of a family of differing Vγ genes variously rearranging to the two γ-chain constant (C) region genes14,15. Here we report the cloning of two very different Vγ genes rearranged to J segments upstream of the two human Cγ genes. Both Vγ genes are rearranged productively but nucleotide sequence comparison shows that they possess very little homology with each other. This shows that human T-cell Vγ genes exist which differ significantly from each other at the nucleotide level and that such diverse genes can be usefully rearranged in different T cells.
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Lefranc, MP., Forster, A. & Rabbitts, T. Rearrangement of two distinct T-cell γ-chain variable-region genes in human DNA. Nature 319, 420–422 (1986). https://doi.org/10.1038/319420a0
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DOI: https://doi.org/10.1038/319420a0
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