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Cyclic GMP-sensitive conductance in outer segment membrane of catfish cones

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Abstract

A cyclic GMP-sensitive conductance has recently been observed with patch-clamp recording in excised inside-out patches of plasma membrane from frog and toad rod outer segments1,2. This conductance has properties suggesting that it is probably the light-sensitive conductance involved in visual transduction1,2. We now report a similar conductance in the outer segment membrane of catfish cones. Cyclic GMP showed positive cooperatively in opening this conductance, with a Hill coefficient of 1.6–3.0 and a half-saturating cGMP concentration of 35–70 µM. Cyclic AMP at 1 mM, or changing Ca concentration (in the presence of Mg), had little effect on the conductance. In physiological solutions the cGMP-induced current had a reversal potential near +10 mV; the current amplitude increased roughly exponentially with membrane potential in both depolarizing and hyperpolarizing directions. Our results suggest that cGMP is also the internal transmitter for phototransduction in cones.

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References

  1. Fesenko, E. E., Kolesnikov, S. S. & Lyubarsky, A. L. Nature 313, 310–313 (1985).

    Article  ADS  CAS  Google Scholar 

  2. Nakatani, K. & Yau, K.-W. Biophys. J. 47, 356a (1985).

    Google Scholar 

  3. Hamill, O. P., Marty, A., Neher, E., Sakmann, E. & Sigworth, F. J. Eur. J. Physiol. 391, 85–100 (1981).

    Article  CAS  Google Scholar 

  4. Detwiler, P. B., Conner, J. D. & Bodoia, R. D. Nature 300, 59–61 (1982).

    Article  ADS  CAS  Google Scholar 

  5. Gray, P. & Attwell, D. Proc. R. Soc. B223, 379–388 (1985).

    ADS  CAS  Google Scholar 

  6. Zimmerman, A. L. & Baylor, D. A. Biophys. J. 47, 357a (1985).

    Article  Google Scholar 

  7. Hodgkin, A. L., McNaughton, P. A., Nunn, B. J. & Yau, K.-W. J. Physiol., Lond. 350, 649–680 (1984).

    Article  CAS  Google Scholar 

  8. Bader, C. R., MacLeish, P. R. & Schwartz, E. A. J. Physiol., Lond. 296, 1–26 (1979).

    Article  CAS  Google Scholar 

  9. Baylor, D. A. & Nunn, B. Biophys. J. 41, 125a (1983).

    Google Scholar 

  10. Bodoia, R. D. & Detwiler, P. B. J. Physiol., Lond. (in the press).

  11. Attwell, D., Werblin, F. S. & Wilson, M. J. Physiol., Lond. 328, 259–283 (1982).

    Article  CAS  Google Scholar 

  12. Jack, J. J. B., Noble, D. & Tsien, R. W. Electric Current Flow in Excitable Cells (Clarendon, Oxford, 1975).

    Google Scholar 

  13. Baylor, D. A., Hodgkin, A. L. & Lamb, T. D. J. Physiol., Lond. 242, 685–727 (1974).

    Article  CAS  Google Scholar 

  14. Baylor, D. A. & Hodgkin, A. L. J. Physiol., Lond. 234, 163–198 (1973).

    Article  CAS  Google Scholar 

  15. Schnapf, J. L. & McBurney, R. N. Nature 287, 239–241 (1980).

    Article  ADS  CAS  Google Scholar 

  16. Detwiler, P. B., Hodgkin, A. L. & McNaughton, P. A. J. Physiol., Lond. 300, 213–250 (1980).

    Article  CAS  Google Scholar 

  17. Nowak, L., Bregestovski, P., Ascher, P., Herbert, A. & Prochiantz, A. Nature 307, 462–465 (1984).

    Article  ADS  CAS  Google Scholar 

  18. Lansman, J. B., Hess, P. & Tsien, R. W. Biophys. J. 47, 67a (1985).

    Google Scholar 

  19. Mayer, M. L., Westbrook, G. L. & Guthrie, P. B. Nature 309, 261–263 (1984).

    Article  ADS  CAS  Google Scholar 

  20. De Vries, G. W., Cohen, A. I., Lowry, O. H. & Ferrendelli, J. A. Expl Eye Res. 29, 315–321 (1979).

    Article  CAS  Google Scholar 

  21. Berger, S. J. et al. J. biol. Chem. 255, 3128–3133 (1980).

    CAS  PubMed  Google Scholar 

  22. Farber, D. B., Souza, D. W., Chase, D. G. & Lolley, R. N. Invest. Ophthal. vis. Sci. 20, 24–31 (1981).

    CAS  PubMed  Google Scholar 

  23. Farber, D. B. & Bok, D. Invest. Ophthal. vis. Sci. 26 Suppl.334 (1985).

    Google Scholar 

  24. Ortez, R. A., Tamayo, A., Johnson, C. & Sperling, H. G. J. Histochem. Cytochem. 31, 1305–1311 (1983).

    Article  CAS  Google Scholar 

  25. Hurwitz, R.L., Bunt-Milam, A. H., Chang, M. L. & Beavo, J. A. J. boil. Chem. 260, 568–573 (1985).

    CAS  Google Scholar 

  26. Levine, J. S. & MacNichol, E. F. Sensory Processes 3, 95–131 (1979).

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas, 77550, USA

    Lawrence Haynes & King-Wai Yau

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  1. Lawrence Haynes
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  2. King-Wai Yau
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Haynes, L., Yau, KW. Cyclic GMP-sensitive conductance in outer segment membrane of catfish cones. Nature 317, 61–64 (1985). https://doi.org/10.1038/317061a0

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  • DOI: https://doi.org/10.1038/317061a0

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