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Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer

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Abstract

We have introduced a full-length complementary DNA of the T-cell antigen receptor β-chain into a mutant human T-cell line that lacked a complete β-chain messenger RNA, had a diminished level of α-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected β-chain led to a normal level of α-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.

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Author notes

  1. Peter Van den Elsen and Cox Terhorst: Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA

  2. Andrew F. Calman, John D. Stobo and Arthur Weiss: The Howard Hughes Institute, Department of Medicine, University of California at San Francisco, California 94143, USA

Authors and Affiliations

  1. The Ontario Cancer Institute and Departments of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, Toronto, Ontario, Canada, M4X 1K9

    Pamela S. Ohashi, Tak W. Mak, Peter Van den Elsen, Yusuke Yanagi, Yasunobu Yoshikai, Andrew F. Calman, Cox Terhorst, John D. Stobo & Arthur Weiss

Authors
  1. Pamela S. Ohashi
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  2. Tak W. Mak
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  3. Peter Van den Elsen
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  4. Yusuke Yanagi
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  5. Yasunobu Yoshikai
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  6. Andrew F. Calman
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  7. Cox Terhorst
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  8. John D. Stobo
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  9. Arthur Weiss
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Ohashi, P., Mak, T., Van den Elsen, P. et al. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. Nature 316, 606–609 (1985). https://doi.org/10.1038/316606a0

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  • DOI: https://doi.org/10.1038/316606a0

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