Skip to main content
SpringerLink
Log in

Sequence of a cDNA clone encoding human preproinsulin-like growth factor II

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

The insulin-like growth factors (IGF) I and II are single-chain serum proteins of 70 and 67 amino acids, respectively, which are synthesized by the liver and possibly other tissues1–4. They are probably required for normal fetal and postnatal growth and development5–11. They also stimulate the growth of cultured cells, possibly by controlling the progression through the G1 phase of the cell cycle12. In contrast to IGF-II whose concentration does not vary during postnatal development, the serum levels of IGF-I increase several-fold to adult levels during puberty13. The serum concentration of IGF-I is a sensitive monitor of growth hormone levels and is decreased in individuals with growth hormone deficiency and elevated in those with growth hormone-secreting tumours8,14. As a first step in studying the biosynthesis of these proteins and elucidating their role(s) in normal development and in tumorigenesis, we have isolated and sequenced cDNAs prepared from adult human liver mRNA which encode the precursors to IGF-I and -II. We report here the sequence of a cDNA encoding a 180-amino acid protein which is the precursor to IGF-II.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Rinderkneckt, E. & Humbel, R. E. J. biol. Chem. 253, 2769–2776 (1978).

    Google Scholar 

  2. Rinderkneckt, E. & Humbel, R. E. FEBS Lett. 89, 283–286 (1978).

    Article  Google Scholar 

  3. Spencer, E. M. (ed.) Insulin-Like Growth Factors/ Somatomedins, Basic Chemistry, Biology, Clinical Importance (ed. Spencer, E. M.) (Walter de Gruyter, New York, 1983).

  4. D'Ercole, A. J., Stiles, A. D. & Underwood, L. E. Proc. natn. Acad. Sci. U.S.A. 81, 935–939 (1984).

    Article  ADS  CAS  Google Scholar 

  5. Phillips, L. S. & Vassiloupoulou-Sellin, R. New. Engl. J. Med. 302, 371–380 (1980).

    Article  CAS  Google Scholar 

  6. Phillips, L. S. & Vassiloupoulou-Sellin, R. New Engl. J. Med. 302, 438–446 (1980).

    Article  CAS  Google Scholar 

  7. Daughaday, W. H. Proc. Soc. exp. Biol. Med. 170, 257–263 (1983).

    Article  Google Scholar 

  8. Merimee, T. J., Zapf, J. & Froesch, E. R. New Engl. J. Med. 305, 965–968 (1981).

    Article  CAS  Google Scholar 

  9. Schoenle, E., Zapf, J., Humbel, R. E. & Froesch, E. R. Nature 296, 252–253 (1982).

    Article  ADS  CAS  Google Scholar 

  10. Salmon, W. D. & Daughaday, W. H. J. Lab. clin. Med. 49, 825–836 (1957).

    CAS  PubMed  Google Scholar 

  11. Moses, A. C. et al. Proc. natn. Acad. Sci. U.S.A. 77, 3649–3653 (1980).

    Article  ADS  CAS  Google Scholar 

  12. Bockus, B. J., Chaikin, M. A. & Stiles, C. D. in Insulin-Like Growth Factors/ Somatomedins, Basic Chemistry, Biology, Clinical Importance (ed. Spencer, E. M.) 381–419 (Walter de Gruyter, New York, 1983).

    Google Scholar 

  13. Clemmons, D. R. & Van Wyk, J. J. in Tissue Growth Factors (ed. Baserga, R.) 161–208 (Springer, Berlin, 1981).

    Book  Google Scholar 

  14. Clemmons, D. R. et al. New Engl. J. Med. 301, 1138–1142 (1979).

    Article  CAS  Google Scholar 

  15. Woods, D. E., Markham, A. F., Ricker, A. T., Goldberger, G. & Collen, H. R. Proc. natn. Acad. Sci. U.S.A. 79, 5661–5665 (1982).

    Article  ADS  CAS  Google Scholar 

  16. Jansen, M. et al. Nature 306, 609–611 (1983).

    Article  ADS  CAS  Google Scholar 

  17. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  18. Hopp, T. P. & Woods, K. R. Proc. natn. Acad. Sci. U.S.A. 78, 3824–3828 (1981).

    Article  ADS  CAS  Google Scholar 

  19. Marquardt, H., Todaro, G. J., Henderson, L. E. & Oroszlan, S. J. biol. Chem. 256, 6859–6865 (1981).

    CAS  Google Scholar 

  20. Moses, A. C., Nissley, S. P., Short, P. A., Rechler, M. M. & Podskalny, J. M. Eur. J. Biochem. 103, 387–400 (1980).

    Article  CAS  Google Scholar 

  21. Acquaviva, A. M., Bruni, C. B., Nissley, S. P. & Rechler, M. M. Diabetes 31, 656–658 (1982).

    Article  CAS  Google Scholar 

  22. Yang, Y. W.-H. et al. in Insulin-Like Growth Factors/ Somatomedins, Basic Chemistry, Biology, Clinical Importance (ed. Spencer, E. M.) 603–610 (Walter de Gruyter, New York, 1983).

    Google Scholar 

  23. Scott, J. et al. Science 221, 236–240 (1982).

    Article  ADS  Google Scholar 

  24. Gubler, U. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4311–4314 (1983).

    Article  ADS  CAS  Google Scholar 

  25. Mayo, K. E., Vale, W., Rivier, J., Rosenfeld, M. G. & Evans, R. M. Nature 306, 86–88 (1983).

    Article  ADS  CAS  Google Scholar 

  26. Steiner, D. F., Quinn, P. S., Chan, S. J., Marsh, J. & Tager, H. S. Ann. N. Y. Acad. Sci. 343, 1–16 (1980).

    Article  ADS  CAS  Google Scholar 

  27. Steiner, D. F. Diabetes 27 (Suppl. 1), 145–148 (1978).

    Article  CAS  Google Scholar 

  28. Dayhoff, M. O. Atlas of Protein Sequence and Structure 150–151 (National Biomedical Research Foundation, Washington, 1978).

    Google Scholar 

  29. Chan, S. J., Kwok, S. C.M. & Steiner, D. F. Diabetes Care 4, 4–10 (1981).

    Article  CAS  Google Scholar 

  30. de Haen, C., Swanson, E. & Teller, D. C. J. molec. Biol. 106, 639–661 (1976).

    Article  CAS  Google Scholar 

  31. Hudson, P. et al. Nature 301, 628–631 (1983).

    Article  ADS  CAS  Google Scholar 

  32. Gergen, J. P., Stern, R. H. & Wensink, P. C. Nucleic Acids Res. 7, 2115–2136 (1979).

    Article  CAS  Google Scholar 

  33. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  34. Denhardt, D. Biochem. biophys. Res. Commun. 23, 641–646 (1966).

    Article  CAS  Google Scholar 

  35. Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H. & Roe, B. A. J. molec. Biol. 143, 161–178 (1980).

    Article  CAS  Google Scholar 

  36. Bell, G. I. et al. Nature 282, 525–527 (1979).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chiron Corporation, 4560 Horton Street, Emeryville, California, 94608, USA

    Graeme I. Bell, James P. Merryweather, Ray Sanchez-Pescador, Michelle M. Stempien & Leslie B. Rall

  2. Molecular Medicine Group, MRC Clinical Research Centre, Harrow, Middlesex, HA1 3UJ, UK

    Linda Priestley & James Scott

Authors
  1. Graeme I. Bell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James P. Merryweather
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ray Sanchez-Pescador
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michelle M. Stempien
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Linda Priestley
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. James Scott
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Leslie B. Rall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bell, G., Merryweather, J., Sanchez-Pescador, R. et al. Sequence of a cDNA clone encoding human preproinsulin-like growth factor II. Nature 310, 775–777 (1984). https://doi.org/10.1038/310775a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/310775a0

  • Springer Nature Limited

This article is cited by

Search

Navigation