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Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse

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Abstract

An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact organisms and provides a system whereby the expression and regulation of cloned genes can be followed within the context of a well characterized developmental programme.

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Authors and Affiliations

  1. Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge

    David A. Williams, Ihor R. Lemischka & Richard C. Mulligan

  2. Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, 02139, USA

    David A. Williams, Ihor R. Lemischka & Richard C. Mulligan

  3. Divisions of Hematology-Oncology, Children's Hospital, Pediatric Oncology, Dana-Farber Cancer Institute and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA

    David A. Williams & David G. Nathan

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  1. David A. Williams
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  2. Ihor R. Lemischka
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  3. David G. Nathan
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  4. Richard C. Mulligan
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Williams, D., Lemischka, I., Nathan, D. et al. Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse. Nature 310, 476–480 (1984). https://doi.org/10.1038/310476a0

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