Abstract
The commitment of non-dividing cells (in G0) to enter division can be studied using primary cultures of lymphocytes. The cells are stable in G0 unless stimulated by mitogen such as concanavalin A (Con A). Commitment to enter the division cycle depends on the expression of gene(s) induced by Con A1,2 and the synthesis of p53 protein correlates with this commitment step3. I show here that in unstimulated cells, a second form of p53 is synthesized and is restricted to G0.
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Milner, S. M. Nature 268, 441–442 (1977).
Milner, J. Nature 275, 660–661 (1978).
Milner, J. & Milner, S. Virology 112, 785–788 (1981).
Milner, J. & McCormick, F. Cell Biol. int. Rep. 4, 663–667 (1980).
Harlow, E., Crawford, L. V., Pim, D. C. & Williamson, N. M. Virology 39, 681–689 (1981).
Gurney, E. G., Harrison, R. O. & Fenno, J. J. Virol. 34, 752–753 (1980).
Yewdell, J., Gannon, J. & Lane, D. P. EMBO J. (submitted).
Coffman, R. L. & Weissman, I. L. J. exp. Med. 153, 269–279 (1981).
Dippold, W. G., Jay, G., DeLeo, A. B., Khoury, G. & Old, L. Proc. natn. Acad. Sci. U.S.A. 78, 1695–1699 (1981).
Ling, N. R. & Kay, J. E. Lymphocyte Stimulation (North-Holland, Amsterdam, 1975).
Benchimol, S., Pim, D. & Crawford, L. V. EMBO J. 1, 1055–1062 (1982).
McCormick, F. & Harlow, E. J. Virol. 34, 213–224 (1980).
Oren, M., Maltzman, W. & Levine, A. J. Molec. cell. Biol. 2, 101–110 (1981).
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Milner, J. Different forms of p53 detected by monoclonal antibodies in non-dividing and dividing lymphocytes. Nature 310, 143–145 (1984). https://doi.org/10.1038/310143a0
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DOI: https://doi.org/10.1038/310143a0
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