Skip to main content
SpringerLink
Log in

Immunoglobulin heavy-chain class switching in a pre-B cell line is accompanied by DNA rearrangement

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Switching of an Abelson virus-transformed mouse lymphoid cell line from immunoglobulin μ to γ2b heavy-chain synthesis in vitro is accompanied by loss of DNA sequences between the JH and Cγ2b gene segments, and thus cannot be explained by differential RNA processing. The light-chain loci of both μ- and γ2b-producing cell clones are in the embryonic configuration, which indicates that class switching can occur in pre-B cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Raff, M. D., Megson, M., Owen, J. J. T. & Cooper, M. D. Nature 259, 224–226 (1976).

    Article  CAS  ADS  Google Scholar 

  2. Burrows, P. D., LeJeune, M. & Kearney, J. F. Nature 280, 838–841 (1979).

    Article  CAS  ADS  Google Scholar 

  3. Marcu, K. B. Cell 29, 719–721 (1982).

    Article  CAS  Google Scholar 

  4. Wabl, M. R. et al. Proc. natn. Acad. Sci. U.S.A. 77, 6793–6796 (1980).

    Article  CAS  ADS  Google Scholar 

  5. Maki, R. et al. Cell 24, 353–365 (1981).

    Article  CAS  Google Scholar 

  6. Abney, E. R., Cooper, M. D., Kearney, J. F., Lawton, A. R. & Parkhouse, R. M. E. J. Immun. 120, 2041–2049 (1978).

    CAS  Google Scholar 

  7. Pernis, B., Forni, L. & Luzzati, A. L. Cold Spring Harb. Symp. quant. Biol. 41, 175–183 (1976).

    Article  Google Scholar 

  8. Yaoita, Y., Kumagai, Y., Okumura, K. & Honjo, T. Nature 297, 697–699 (1982).

    Article  CAS  ADS  Google Scholar 

  9. Shimizu, A., Takahashi, N., Yaoita, Y. & Honjo, T. Cell 28, 499–506 (1982).

    Article  CAS  Google Scholar 

  10. Siden, E. J., Baltimore, D., Clark, D. & Rosenberg, N. E. Cell 16, 389–396 (1979).

    Article  CAS  Google Scholar 

  11. Burrows, P. D., Beck, G. B. & Wabl, M. R. Proc. natn. Acad. Sci. U.S.A. 78, 564–568 (1981).

    Article  CAS  ADS  Google Scholar 

  12. Alt, F. W., Rosenberg, N., Casanova, R. J., Thomas, E. & Baltimore, D. Nature 296, 325–331 (1982).

    Article  CAS  ADS  Google Scholar 

  13. Riley, S. C., Brock, E. J. & Kuehl, W. M. Nature 289, 804–806 (1981).

    Article  CAS  ADS  Google Scholar 

  14. Wabl, M. R., Beck-Engeser, G. B. & Burrows, P. D. Proc. natn. Acad. Sci. U.S.A. (submitted).

  15. Maki, R., Kearney, J., Paige, C. & Tonegawa, S. Science 209, 1366–1369 (1980).

    Article  CAS  ADS  Google Scholar 

  16. Vogler, L. B. et al. Nature 290, 339–341 (1981).

    Article  CAS  ADS  Google Scholar 

  17. Nossal, G. J. V., Warner, N. & Lewis, H. Cell. Immun. 2, 41–53 (1971).

    Article  CAS  Google Scholar 

  18. Kearney, J. F., Cooper, M. D. & Lawton, A. R. J. Immun. 116, 1664 (1976).

    CAS  PubMed  Google Scholar 

  19. Wabl, M. R., Forni, L. & Loor, F. Science 199, 1078–1080 (1978).

    Article  CAS  ADS  Google Scholar 

  20. Kearney, J. F., Radbruch, A., Liesegang, B. & Rajewsky, K. J. Immun. 123, 1548–1550 (1979).

    CAS  Google Scholar 

  21. Liu, C. P., Tucker, P. W., Mushinski, J. F. & Blattner, F. R. Science 209, 1348–1353 (1980).

    Article  CAS  ADS  Google Scholar 

  22. Sakano, H., Maki, R., Kurosawa, Y., Roeder, W. & Tonegawa, S. Nature 286, 676–683 (1980).

    Article  CAS  ADS  Google Scholar 

  23. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  24. Gross-Bellard, M., Dudet, P. & Chambon, P. Eur. J. Biochem. 36, 32–38 (1973).

    Article  CAS  Google Scholar 

  25. Tucker, P. W., Marcu, K. B., Newell, N., Richards, J. & Blattner, F. R. Science 206, 1303–1306 (1979).

    Article  CAS  ADS  Google Scholar 

  26. Yaoita, Y. & Honjo, T. Nature 286, 850–853 (1980).

    Article  CAS  ADS  Google Scholar 

  27. Kataoka, T., Miyata, T. & Honjo, T. Cell 23, 357–368 (1981).

    Article  CAS  Google Scholar 

  28. Tucker, P. W., Marcu, K. B., Slightom, J. L. & Blattner, F. R. Science 206, 1299–1303 (1979).

    Article  CAS  ADS  Google Scholar 

  29. Hozumi, N., Hawley, R. G. & Murialdo, H. Gene 13, 163–172 (1981).

    Article  CAS  Google Scholar 

  30. Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).

    Article  CAS  ADS  Google Scholar 

  31. Sakano, H., Hüppi, K., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).

    Article  CAS  ADS  Google Scholar 

  32. Brack, C., Hirama, M., Lenhard-Schuller, R. & Tonegawa, S. Cell 15, 1–14 (1978).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Spemannstrasse 37–39, D-7400, Tübingen, FRG

    Peter D. Burrows, Gabriele B. Beck-Engeser & Matthias R. Wabl

Authors
  1. Peter D. Burrows
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gabriele B. Beck-Engeser
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Matthias R. Wabl
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Burrows, P., Beck-Engeser, G. & Wabl, M. Immunoglobulin heavy-chain class switching in a pre-B cell line is accompanied by DNA rearrangement. Nature 306, 243–246 (1983). https://doi.org/10.1038/306243a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/306243a0

  • Springer Nature Limited

This article is cited by

Search

Navigation