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Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon

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Abstract

T-lymphocyte-mediated responses to the cellular components of blood vessels are important in rejection of allografts1–3. The induction of cytolytic T lymphocytes (CTLs) depends on recognition of foreign class II major histocompatibility complex antigens (human HLA-DR, DC/DS, SB and others, collectively referred to as Ia) on the target cells whereas killing by CTLs usually depends on recognition of foreign class I antigens (HLA-A,B)4, although some alloreactive CTLs recognize foreign Ia instead of HLA-A, B (refs 5–8). The expression of Ia antigens has traditionally been regarded as restricted to immunological cell types, and the presence of class II antigen-bearing ‘passenger’ leukocytes in rodent organ grafts appears necessary for graft rejection9–11. Recently, Ia antigens have been observed by immunofluorescence microscopy on human renal and dermal capillary endothelium12–15. We have previously shown that human umbilical vein endothelial (HUVE) cells in standard culture conditions do not bear Ia antigens, but may be induced to do so by products of lectin- or alloantigen-activated T lymphocytes16,17. Furthermore, we found that recombinant immune interferon (IFN-γ), free of other lymphokines, is a potent inducer of Ia expression in HUVE cells17. Here we report that IFN-γ also induces Ia expression on human foreskin capillary endothelial (HFCE) cells, HUVE cells transformed by Simian virus 40 viral DNA (SV-HUVE cells) and human dermal fibroblast (HDF) cells in culture. Further, we present evidence that Ia present on HUVE cells and HDF cells can be functionally recognized by human T cells, resulting in a two-way interaction between T cells and mesenchymal cells that may be important in allograft rejection.

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Authors and Affiliations

  1. Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, 02115, USA

    Jordan S. Pober, Tucker Collins, Michael A. Gimbrone Jr & Ramzi S. Cotran

  2. Department of Pediatric Medicine, Children's Hospital Medical Center, Boston, Massachusetts, 02115, USA

    Jonathan D. Gitlin

  3. Department of Pediatric Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts, 02115, USA

    Carol Clayberger, Alan M. Krensky, Steven J. Burakoff & Carol S. Reiss

  4. The Laboratory of Molecular Biology, State University of Ghent, Ghent, Belgium

    Walter Fiers

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  1. Jordan S. Pober
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  2. Tucker Collins
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  7. Carol Clayberger
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Pober, J., Collins, T., Gimbrone, M. et al. Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. Nature 305, 726–729 (1983). https://doi.org/10.1038/305726a0

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  • DOI: https://doi.org/10.1038/305726a0

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