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Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAA

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Abstract

A single U → G transversion in the 3′ consensus sequence AAUAAA of the adenovirus early region 1A gene was constructed and the effect of this mutation on processing of the 3′ end of the nuclear early region 1A RNAs was analysed. The results demonstrate that the intact AAUAAA is not required for RNA polyadenylation but is required for the cleavage step preceding polyadenylation to occur efficiently.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Biology Institute, University of California, Los Angeles, California, 90024, USA

    Craig Montell & Arnold J. Berk

  2. Department of Chemistry, University of Colorado, Campus Box 215, Boulder, Colorado, 80309, USA

    Eric F. Fisher & Marvin H. Caruthers

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  1. Craig Montell
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  4. Arnold J. Berk
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Montell, C., Fisher, E., Caruthers, M. et al. Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAA. Nature 305, 600–605 (1983). https://doi.org/10.1038/305600a0

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