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Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3

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Abstract

The three serotypes of poliovirus are members of the picornaviradae, a group of viruses which cause a variety of diseases of considerable importance in man and animals. We have previously used antigenic mutants resistant to neutralizing monoclonal antibodies to identify a single antigenic site for the neutralization of poliovirus type 3 (ref. 1). Evidence based on oligonucleotide mapping suggested that this site corresponded largely to one physicochemical region of the capsid protein viral polypeptide 1 (VP1). We now present conclusive evidence that most of the mutations conferring resistance to neutralization are confined to an eight-amino acid region of VP1, specified by a sequence of viral RNA 277–300 bases from the start of the region coding for VP1. These data strongly suggest that this small region constitutes a major antigenic site involved in virus neutralization and they provide the most detailed information currently available on the antigenic site of a human virus.

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Authors and Affiliations

  1. National Institute for Biological Standards and Control, Holly Hill, London, NW3 6RB, UK

    D. M. A. Evans, P. D. Minor & G. S. Schild

  2. Department of Microbiology, University of Leicester, Leicester, LE1 7RH, UK

    J. W. Almond

Authors
  1. D. M. A. Evans
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  2. P. D. Minor
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  3. G. S. Schild
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  4. J. W. Almond
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Evans, D., Minor, P., Schild, G. et al. Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3. Nature 304, 459–462 (1983). https://doi.org/10.1038/304459a0

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  • DOI: https://doi.org/10.1038/304459a0

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