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Direct evidence for microfilament-mediated capping of surface receptors on crawling fibroblasts

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Abstract

On moving fibroblasts, cell-surface receptors cross-linked by antibodies or lectins are cleared centripetally from regions of lamellar cytoplasm and collect as a cap over the perinuclear region1–4. Current theories of the mechanism of receptor redistribution on cultured cells variously implicate membrane flow5–7, lipid flow8, surface waves9–10 and linkage to the cytoskeleton11–13. The last, the anchorage model13, is based on observations that ligand-induced clusters of receptors on a variety of cell types either attach to actin14 or align over structures containing actin 15–17, myosin15,16and α-actinin18 .I show here that the capping of antibody receptors on crawling chick embryo fibroblasts is highly coordinated with the apparent centripetal movements of arcs19–22, which are part of a dorsal cortical actin–microfilament sheath (DCMS). This phenomenon can be directly observed in living cells. The data support the anchorage model of membrane receptor mobility and suggest that there is a continuous flow of actin associated with fibroblast locomotion.

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  1. MRC Cell Biophysics Unit, Department of Biophysics, King's College, 26–29 Drury Lane, London, WC2B 5RL, UK

    Julian P. Heath

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  1. Julian P. Heath
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Heath, J. Direct evidence for microfilament-mediated capping of surface receptors on crawling fibroblasts. Nature 302, 532–534 (1983). https://doi.org/10.1038/302532a0

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