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Structure and expression of a cloned cDNA for human interleukin-2

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Abstract

A cDNA coding for human interleukin-2 (IL-2) has been cloned from a cDNA library prepared from partially purified IL-2 mRNA. The DNA sequence codes for a polypeptide which consists of 153 amino acids including a putative signal sequence. A biologically active polypeptide, characteristic of human IL-2, was produced when the cDNA was fused to a simian virus 40 promoter sequence and used to transfect cultured monkey COS cells.

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Author notes

  1. Nobukazu Kashima, Ryota Yoshimoto and Junji Hamuro: Central Research Laboratories, Ajinomoto Co. Inc., Totsuka-ku, Yokohama 244, Japan

Authors and Affiliations

  1. Department of Biochemistry, Cancer Institute, Japanese Foundation for Cancer Research, Toshima-ku, Tokyo, 170, Japan

    Tadatsugu Taniguchi, Hiroshi Matsui, Takashi Fujita, Chikako Takaoka, Nobukazu Kashima, Ryota Yoshimoto & Junji Hamuro

Authors
  1. Tadatsugu Taniguchi
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  2. Hiroshi Matsui
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  3. Takashi Fujita
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  4. Chikako Takaoka
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  5. Nobukazu Kashima
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  6. Ryota Yoshimoto
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  7. Junji Hamuro
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Taniguchi, T., Matsui, H., Fujita, T. et al. Structure and expression of a cloned cDNA for human interleukin-2. Nature 302, 305–310 (1983). https://doi.org/10.1038/302305a0

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  • DOI: https://doi.org/10.1038/302305a0

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