Abstract
The genetic change that leads to the activation of the oncogene in T24 human bladder carcinoma cells is shown to be a single point mutation of guanosine into thymidine. This substitution results in the incorporation of valine instead of glycine as the twelfth amino acid residue of the T24 oncogene-encoded p21 protein. Thus, a single amino acid substitution appears to be sufficient to confer transforming properties on the gene product of the T24 human bladder carcinoma oncogene.
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Cooper, G. M. Science 217, 801–806 (1982).
Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 78, 1181–1184 (1981).
Shih, C., Padhy, C., Murray, M. & Weinberg, R. A. Nature 290, 261–264 (1981).
Lane, M. A., Sainten, A. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 78, 5185–5189 (1981).
Murray, M. J. et al. Cell 25, 355–361 (1981).
Perucho, M. et al. Cell 27, 467–476 (1981).
Lane, M. A., Sainten, A. & Cooper, G. M. Cell 28, 873–880 (1982).
Pulciani, S. et al. Proc. natn. Acad. Sci. U.S.A. 79, 2845–2849 (1982).
Goldfarb, M., Shimizu, K., Perucho, M. & Wigler, M. Nature 296, 404–409 (1982).
Shih, C. & Weinberg, R. A. Cell 29, 161–169 (1982).
Santos, E., Tronick, S. R., Aaronson, S. A., Pulciani, S. & Barbacid, M. Nature 298, 343–347 (1982).
Der, C. J., Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 79, 3637–3640 (1982).
Parada, L. F., Tabin, C. J., Shih, C. & Weinberg, R. A. Nature 297, 474–478 (1982).
Chang, E. H., Gonda, M. A., Ellis, R. W., Scolnick, E. M. & Lowy, D. R. Proc. natn. Acad. Sci. U.S.A. 79, 4848–4852 (1982).
Dhar, R. et al. Science 217, 934–937 (1982).
Reddy et al. (in preparation).
Sharp, P. A. Cell 23, 643–646 (1981).
Tsuchida, N., Ryder, T. & Ohtsubo, E. Science 217, 937–939 (1982).
Pulciani, S., Santos, E., Lauver, A. V., Long, L. K. & Barbacid, M. Nature (in the press).
Garnier, J., Osguthorpe, D. J. & Robson, B. J. molec. Biol. 120, 97–120 (1978).
Shih, T. Y. et al. J. Virol. 42, 253–261 (1982).
Chang, E. H., Furth, M. E., Scolnick, E. M. & Lowy, D. R. Nature 297, 479–483 (1982).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
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Reddy, E., Reynolds, R., Santos, E. et al. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene. Nature 300, 149–152 (1982). https://doi.org/10.1038/300149a0
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DOI: https://doi.org/10.1038/300149a0
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