Abstract
We have used a probe (pAS-1) to the human urea cycle enzyme argininosuccinate synthetase (AS) to detect individual variation in restriction enzyme digests of human DNA from lymphocytes, fibroblasts and transformed cell lines (including HeLa). pAS-1 is a 1.55 kilobase (kb) sequence of cDNA from poly(A)+ RNA cloned in pBR322; it represents more than 90% of the AS message1. At least 15 genomic fragments are detected with pAS-1 in DNA digested with restriction enzymes recognizing 6-base sequences2. This multiplicity of fragments is explained in part by the observation that AS-like sequences are found on more than seven human chromosomes3. We report here population data and gene dosage experiments demonstrating an AS-like sequence on the human Y chromosome and confirming an AS-like sequence on the human X chromosome.
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Su, T.-S., Bock, H.-G., O'Brien, W. E. & Beaudet, A. L. J. biol. Chem. 256, 11826–11831 (1981).
Daiger, S. P., Wildin, R. S. & Su, T.-S. Am. J. hum. Genet. 33, 136a (1981).
Beaudet, A. L. et al. Cell (in the press).
Carritt, B. & Povey, S. Cytogenet. Cell Genet. 23, 171–181 (1979).
Meera Khan, P. & Robson, E. B. Cytogenet. Cell Genet. 25, 39–46 (1979).
Rao, D. C. Hum. Hered. 20, 486–492 (1970).
Stern, C., Centerwall, W. R. & Sarkar, S. S. Am. J. hum. Genet. 16, 455–471 (1964).
Wachtel, S. S., Ohno, S., Koo, G. C. & Boyse, E. A. Nature 257, 235–236 (1975).
Wolf, U. Hum. Genet. 58, 25 (1981).
McKusick, V. M. Mendelian Inheritance in Man, 5th edn, 1xxiv, (Johns Hopkins Press, Boston, 1978).
Bobrow, M., Pearson, P. L., Pike, M. C. & El-Alfo, O. Cytogenetics 10, 190–198 (1971).
Kunkel, L. M., Smith, K. D. & Boyer, S. H. Science 191, 1189–1190 (1976).
Cooke, H. Nature 262, 182–186 (1976).
Davies, K. E., Young, B. D., Elles, R. G., Hill, M. E. & Williamson, R. Nature 293, 374–376 (1981).
Nishioka, Y., Leder, A. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 77, 2806–2809 (1980).
Vanin, E. F., Goldberg, G. I., Tucker, P. W. & Smithies, O. Nature 286, 222–226 (1980).
Kan, Y. W., Dozey, A. M., Trecartin, R. & Todd, D. New Engl. J. Med. 297, 1081–1084 (1977).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
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Daiger, S., Wildin, R. & Su, TS. Sequences on the human Y chromosome homologous to the autosomal gene for argininosuccinate synthetase. Nature 298, 682–684 (1982). https://doi.org/10.1038/298682a0
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DOI: https://doi.org/10.1038/298682a0
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