Abstract
Adenosine and its 5′-phosphorylated congeners evoke specific membrane-mediated responses in excitable tissues1,2. Available data suggest that inhibition of the target cell occurs due to hyperpolarization3,4, and in some preparations a compound effect of ATP (excitation and inhibition) has been found5,6. However, the ionic mechanism of the purinergic-mediated response has not been studied by standard intracellular voltage-clamping techniques. Recently, we have discovered purinergic receptors in the Xenopus oocyte, a well defined giant cell amenable to rigorous electrophysiological7 and biochemical8 studies. We report here that in these cells, adenosine-induced slow membrane responses consisted of an early depolarizing (D) transient current carried by Cl− ions, followed by a steady hyperpolarizing (H) current involving K+ ions. The relative potency sequence for the D current was ATP≃ ADP > AMP ≃ adenosine; this order was reversed for the H current.
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References
Burnstock, G. Circulation Res. 46, 1175–1182 (1980).
Urthaler, F., Woods, W. T., James, T. N. & Walker, A. A. J. Pharmac. exp. Ther. 216, 254–260 (1981).
Hartzell, H. C. J. Physiol., Lond. 293, 23–50 (1979).
Tomita, T. & Watanabe, H. J. Physiol., Lond. 231, 167–178 (1973).
Flitney, F. W. & Singh, I. J. Physiol., Lond. 304, 21–42 (1980).
Yatani, A., Goto, M. & Tsuda, Y. Jap. J. Physiol. 28, 47–61 (1978).
Kusano, K., Miledi, R. & Stinnakre, J. Nature 270, 739–741 (1977).
Maller, J. L., Butcher, F. R. & Krebs, E. G. J. biol. Chem. 254, 579–582 (1979).
Dumont, J. N. J. Morph. 136, 153–180 (1972).
Morrill, G. A. & Ziegler, D. Devl Biol. 14, 216–223 (1980).
Burnstock, G. J. Physiol., Lond. 313, 1–34 (1981).
Van Calker, D., Muller, M. & Hamprecht, B. Nature 276, 839–841 (1978).
Londos, C. & Wolff, J. Proc. natn. Acad. Sci. U.S.A. 74, 5482–5486 (1977).
Londos, C., Cooper, D. M. F. & Wolff, J. Proc. natn. Acad. Sci. U.S.A. 77, 2551–2554 (1980).
Brown, C. M. & Burnstock, G. Br. J. Pharmac. 73, 617–624 (1981).
Dascal, N. & Landau, E. M. Life Sci. 27, 1423–1428 (1980).
Rodbell, M. Nature 284, 17–22 (1980).
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Lotan, I., Dascal, N., Cohen, S. et al. Adenosine-induced slow ionic currents in the Xenopus oocyte. Nature 298, 572–574 (1982). https://doi.org/10.1038/298572a0
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DOI: https://doi.org/10.1038/298572a0
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