Skip to main content

Advertisement

Log in

De novo DNA synthesis by a novel mouse DNA polymerase associated with primase activity

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Several studies have shown that short nascent DNAs (Okazaki fragments) of mammalian cells1–3 and papovaviruses4,5 possess an 8–11-nucleotide RNA sequence covalently linked to their 5′ end, suggesting that this RNA sequence acts in priming in DNA synthesis. Although many studies have indicated that DNA polymerase α probably participates in the synthesis of Okazaki fragments6–10, to date, the reconstitution of de novo DNA synthesis by coupled reaction of DNA polymerase and classical RNA polymerases of mammalian cells has been unsuccessful. Here we report that a novel mouse DNA polymerase associated with primase activity11,12 synthesized DNA of ∼600 nucleotides after synthesis of initiator RNA (iRNA) of 8–10 nucleotides in the presence of a specific stimulating factor. The similarity of this DNA synthesis to that in vivo suggests the involvement of this novel DNA polymerase in the reaction required to initiate synthesis of Okazaki fragments in chromosomal DNA replication.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Tseng, B. Y., Erickson, J. M. & Goulian, M. J. molec. Biol. 129, 531–545 (1979).

    Article  CAS  Google Scholar 

  2. Kowalski, J. & Denhardt, D. T. Nature 281, 704–706 (1979).

    Article  ADS  CAS  Google Scholar 

  3. Tseng, B. Y. & Goulian, M. Cell 12, 483–489 (1977).

    Article  CAS  Google Scholar 

  4. Reichard, P., Eliasson, R. & Söderman, G. Proc. natn. Acad. Sci. U.S.A. 71, 4901–4905 (1974).

    Article  ADS  CAS  Google Scholar 

  5. Kaufmann, G. J. molec. Biol. 147, 25–39 (1981).

    Article  CAS  Google Scholar 

  6. Bertazzoni, V. et al. Proc. natn. Acad. Sci. U.S.A. 73, 785–789 (1976).

    Article  ADS  CAS  Google Scholar 

  7. Hübscher, V., Kuenzle, C. C. & Spadari, S. Nucleic Acids Res. 4, 2917–2929 (1977).

    Article  Google Scholar 

  8. Edenberg, H. J., Anderson, S. & DePamphilis, M. L. J. biol. Chem. 253, 3273–3280 (1978).

    CAS  PubMed  Google Scholar 

  9. Krokan, H., Schaffer, P. & DePamphilis, M. L. Biochemistry 18, 4431–4443 (1979).

    Article  CAS  Google Scholar 

  10. Yagura, T. & Seno, T. Biochim. biophys. Acta 608, 277–286 (1980).

    Article  CAS  Google Scholar 

  11. Yagura, T., Kozu, T. & Seno, T. J. Biochem., Tokyo 91, 607–618 (1982).

    Article  CAS  Google Scholar 

  12. Yagura, T. et al. Biochem. biophys. Res. Commun. 104, 1189–1195 (1982).

    Article  CAS  Google Scholar 

  13. Yagura, T., Kozu, T. & Seno, T. J. biol. Chem. (in the press).

  14. Rowen, L. & Kornberg, A. J. biol. Chem. 253, 770–774 (1978).

    CAS  PubMed  Google Scholar 

  15. Kozu, T., Yagura, T. & Seno, T. Cell Struct. Function 7, 9–19 (1982).

    Article  CAS  Google Scholar 

  16. Tapper, D. P. & Clayton, D. A. Nucleic Acids Res. 9, 6787–6804 (1981).

    Article  CAS  Google Scholar 

  17. Ikeda, J., Longiaru, M., Horwitz, M. S. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 77, 5827–5831 (1980).

    Article  ADS  CAS  Google Scholar 

  18. Burke, J. F., Plummer, J., Hurberman, J. A. & Evans, M. J. Biochim. biophys. Acta 609, 205–223 (1980).

    Article  CAS  Google Scholar 

  19. Villani, G., Fay, P. J., Bambara, R. A. & Lehman, J. R. J. biol. Chem. 256, 8202–8207 (1981).

    CAS  PubMed  Google Scholar 

  20. Pigiet, V., Eliasson, R. & Reichard, P. J. molec. Biol. 84, 197–216 (1974).

    Article  CAS  Google Scholar 

  21. Kaufmann, G. & Falk, H. H. Nucleic Acids Res. (in the press).

  22. Kozu, T., Aida, M., Ikawa, Y. & Seno, T. Eur. J. Cancer 14, 1065–1076 (1978).

    Article  CAS  Google Scholar 

  23. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  24. Donis-Keller, H., Maxam, A. M. & Gilbert, W. Nucleic Acids Res. 4, 2527–2538 (1977).

    Article  CAS  Google Scholar 

  25. Yagura, T., Yagura, M. & Muramatsu, M. J. molec. Biol. 133, 533–547 (1979).

    Article  CAS  Google Scholar 

  26. Chamberlain, J. P. Analyt. Biochem. 98, 132–135 (1979).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kozu, T., Yagura, T. & Seno, T. De novo DNA synthesis by a novel mouse DNA polymerase associated with primase activity. Nature 298, 180–182 (1982). https://doi.org/10.1038/298180a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/298180a0

  • Springer Nature Limited

Navigation