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Malignant transformation of human embryo retinoblasts by cloned adenovirus 12 DNA

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Abstract

Human adenoviruses can morphologically transform rodent cells in vitro. The transforming gene(s) resides in the early region 1 (E1) transcription unit (0–11.2 map units) at the left end of the conventional adenovirus genome1–4. The only adenovirus-transformed human cell line reported so far, the 293 cell line, was isolated from cell cultures transfected with sheared DNA of the non-oncogenic subgroup C adenovirus type 5 (Ad 5)5,6. The ability of this line to complement the growth of Ad 5 E1 mutants has facilitated the isolation of transformation-defective Ad 5 mutants7,8. As no oncogenic subgroup A adenovirus type 12 (Ad 12) transformed human cell lines were available for the selection of Ad 12 E1 mutants, we attempted to isolate such lines by transfecting cell cultures with a recombinant plasmid (pAsc2) which contained the Ad 12 EcoRI-C DNA fragment (0–16.5 map units) inserted at the EcoRI site of pAT153 (ref. 9). We describe here the isolation of a pAsc2-transformed human embryo retinoblast cell line that contains many copies of the Ad 12 transforming region, expresses virus-specified proteins and, following inoculation into athymic mice, forms tumours which resemble retinoblastomas.

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Authors and Affiliations

  1. Cancer Research Campaign Laboratories, Department of Cancer Studies, University of Birmingham, The Medical School, Birmingham, B15 2TJ, UK

    P. Byrd, K. W. Brown & P. H. Gallimore

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  1. P. Byrd
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  2. K. W. Brown
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  3. P. H. Gallimore
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Byrd, P., Brown, K. & Gallimore, P. Malignant transformation of human embryo retinoblasts by cloned adenovirus 12 DNA. Nature 298, 69–71 (1982). https://doi.org/10.1038/298069a0

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