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Superattenuation in the tryptophan operon of Serratia marcescens

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Abstract

Deletions were generated in vitro in the leader region of the tryptophan operon of Serratia marcescens and subsequently incorporated into the Escherichia coli chromosome in single copy form. Deletions which removed the translation start codon for the leader peptide or which ended in the ribosome recognition region preceding the leader peptide coding segment, caused super attenuation, that is, increased transcription termination at the attenuator. Apparently, the capacity to initiate translation of this coding region modulates expression of the operon.

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Author notes

  1. Iwona Stroynowski

    Present address: Division of Biology, California Institute of Technology, Pasadena, California, 91109, USA

Authors and Affiliations

  1. Department of Biological Sciences, Stanford University, Stanford, California, 94305, USA

    Iwona Stroynowski, Magda van Cleemput & Charles Yanofsky

Authors
  1. Iwona Stroynowski
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  2. Magda van Cleemput
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  3. Charles Yanofsky
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Stroynowski, I., van Cleemput, M. & Yanofsky, C. Superattenuation in the tryptophan operon of Serratia marcescens. Nature 298, 38–41 (1982). https://doi.org/10.1038/298038a0

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