Abstract
Transfer of a 36-kilobase piece of human DNA containing the β-interferon (IFN-β) gene into mouse Ltk− cells leads to transient expression of human interferon even without an exogenous inducer. A low level of human interferon expression is also found in most stable clones containing the transferred DNA. With double-stranded RNA or Newcastle disease virus (NDV) as inducer, human interferon expression is greatly increased. The induced transcript is identical to normal human IFN-β mRNA. Neighbouring genes contained on the transferred DNA are co-induced but are not essential for the production of human interferon in mouse L cells.
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Hauser, H., Gross, G., Bruns, W. et al. Inducibility of human β-interferon gene in mouse L-cell clones. Nature 297, 650–654 (1982). https://doi.org/10.1038/297650a0
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DOI: https://doi.org/10.1038/297650a0
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