Abstract
The full three-dimensional structure determination of tomato bushy stunt virus has shown how its protein subunit can accommodate to different packing environments in the viral coat and how the size of the particle is nonetheless determined without ambiguity. Reversible, cooperative expansion of the virus at pH values above neutrality and in the absence of divalent cation requires further bonding alternatives for the subunit. We report here the structure of the expanded tomato bushy stunt virus particle, determined at 8 Å resolution by X-ray crystallography. Subunit conformational changes are localized, and folded domains are essentially invariant. The bonding properties of the subunit can account for controlled polymorphism of the assembly.
Similar content being viewed by others
References
Ziegler, A., Harrison, S. C. and Leberman, R. Virology 59, 509 (1972).
Caspar, D. L. D. & Klug, A., Cold Spring Harb. Symp. quant. Biol. 27, 1 (1962).
Harrison, S. C., Olson, A. J., Schutt, C. E., Winkler, F. K. & Bricogne, G. Nature 276, 368 (1978).
Chauvin, C., Witz, J. & Jacrot, B. J. molec. Biol. 124, 641 (1978).
Munowitz, M. G., Dobson, C. M., Griffin, R. G. & Harrison, S. C. J. molec. Biol. 141, 327 (1980).
Finch, J. T., Klug, A. & Leberman, R. J. molec. Biol. 50, 215 (1970).
Incardona, N. L. & Kaesberg, P. Biophys. J. 4, 11 (1964).
Bancroft, J. B. Adv. Virus Res. 16, 99 (1970).
Caspar, D. L. D. Adv. Protein Chem. 18, 37 (1963).
Hogle, J. M., Kirchhausen, T. & Harrison, S. C. J. molec. Biol. (submitted).
Harrison, S. C. Biophys. J. 32, 139 (1980).
McPherson, A. Meth. Biochem. Analysis 23, 249 (1975)
Bricogne, G. Acta crystallogr. A30, 395 (1974).
Arndt, U. W. & Wonacott, A. J. The Rotation Method in Crystallography (North-Holland, Amsterdam, 1977).
Winkler, F. K., Schutt, C. E., Harrison, S. C. & Bricogne, G. Nature 265, 509 (1977).
Dickerson, R. E., Kendrew, J. C. & Strandberg, B. E. Acta crystallogr. 14, 1188 (1961).
Sorger, P. K., Stockley, P. G., Robinson, I. K. & Harrison, S. C. (in preparation).
Stroud, R. M., Kossiakoff, A. A. & Chambers, J. L. A. Rev. Biophys. Bioengng 6, 177 (1977).
Debye, P. Ann. Phys. 43, 49 (1914).
Abad-Zapatero, C. et al. Nature 286, 33 (1980).
Hsu, C. H., White, J. A. & Sehgal, O. P. Virology 81, 471 (1977).
Pfeiffer, P. & Hirth, L. FEBS Lett. 56, 144 (1975).
Durham, A. C. H., Hendry, D. A. & von Wechmar, M. B. Virology 77, 524 (1977).
Kruse, J. & Witz, J. (in preparation).
Rueckert, R. R. Comp. Virol. 6, 131 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Robinson, I., Harrison, S. Structure of the expanded state of tomato bushy stunt virus. Nature 297, 563–568 (1982). https://doi.org/10.1038/297563a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/297563a0
- Springer Nature Limited
This article is cited by
-
Uncoating Mechanism of Carnation Mottle Virus Revealed by Cryo-EM Single Particle Analysis
Scientific Reports (2015)
-
An M18L24 stellated cuboctahedron through post-stellation of an M12L24 core
Nature Chemistry (2012)
-
A crystallographic approach to structural transitions in icosahedral viruses
Journal of Mathematical Biology (2012)