Abstract
Only a fraction of the genome of adenovirus, a DNA tumour virus, is required for transformation in vitro1–4. The leftmost 11% of the genome, including the two transcription units E1A and E1B, appear to be sufficient for transformation of rat embryo cells in tissue culture for highly, weakly and non-oncogenic adenovirus serotypes5,6. Various spliced mRNAs, orginating from the transforming region, have been identified7-11 and in some cases the corresponding protein products found12–14 but it is unknown what renders some serotypes highly oncogenic for baby hamsters compared with others that are non-oncogenic although able to transform cells in vitro. We have studied the mRNAs from the transforming region of the highly oncogenic serotype 12 (Ad12) in the hope of finding novel mRNAs which could account for the oncogenic properties of the virus. We have previously failed to find any differences between the spliced mRNAs in the transcription unit of the E1A region of Ad12 and the corresponding mRNAs from the non-oncogenic type 2 (Ad2)15. Here we have extended our analysis to the E1B region and show that the highly oncogenic Ad12 encodes mRNAs which have different structures from those encoded by Ad2.
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Virtanen, A., Pettersson, U., Moullec, J. et al. Different mRNAs from the transforming region of highly oncogenic and non-oncogenic human adenoviruses. Nature 295, 705–707 (1982). https://doi.org/10.1038/295705a0
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DOI: https://doi.org/10.1038/295705a0
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