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Expression of cell-surface HLA-DR, HLA-ABC and glycophorin during erythroid differentiation

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Abstract

The unexpected discovery that la-like (HLA-DR) antigens1–3 in humans were present on blast cells from acute myeloblastic leukaemia4,5 led to the finding that normal granulocytic progenitors, in contrast to their mature descendents, also expressed HLA-DR antigens. Thus, anti-Ia sera stain a proportion of myeloblasts in normal bone marrow6,7, inhibit myeloid progenitor (CFU-GM) colony formation in the presence of complement8 and can be used to label and separate CFU-GM on a fluorescence-activated cell sorter (FACS)9. Winchester et al. subsequently reported that erythroid progenitors (BFU-E and CFU-E) were also inhibited or killed by anti-Ia (p28,37) and complement10. These observations raised the possibility that HLA-DR (or presumptive I-region equivalent) products might have a regulatory role in early haematopoiesis. We have now analysed HLA-DR and HLA-ABC antigen expression on normal erythroid progenitors using monoclonal antibodies to non-polymorphic determinants and fluorescence-activated cell sorting. In parallel experiments, we tested a monoclonal antibody to glycophorin, a well defined erythroid-speciflc cell-surface membrane glycoprotein11. We report that HLA-DR, HLA-ABC and glycophorin are all expressed at various stages during erythroid differentiation.

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Authors and Affiliations

  1. Membrane Immunology Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX, UK

    Jean Robinson, Domenico Delia & Melvyn Greaves

  2. Department of Haematology, The Hospital for Sick Children, Great Ormond Street, London, WC1, UK

    Colin Sieff

  3. Ludwig Institute for Cancer Research, Sutton, Surrey, SM2 5PX, UK

    Paul A. W. Edwards

Authors
  1. Jean Robinson
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  2. Colin Sieff
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  3. Domenico Delia
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  4. Paul A. W. Edwards
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  5. Melvyn Greaves
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Robinson, J., Sieff, C., Delia, D. et al. Expression of cell-surface HLA-DR, HLA-ABC and glycophorin during erythroid differentiation. Nature 289, 68–71 (1981). https://doi.org/10.1038/289068a0

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  • DOI: https://doi.org/10.1038/289068a0

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