Abstract
Messenger RNA was covalently linked to 18S ribosomal RNA in eukaryotic 40S and 80S initiation complexes by photoreaction with an RNA cross-linking agent, 4′-substituted psoralen. The sites of interaction of the mRNA capped 5′-region included some 3′-ends of 18S rRNA.
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Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).
Steitz, J. A. & Jakes, K. Proc. natn. Acad. Sci. U.S.A. 72, 4734–4738 (1975).
Dunn, J. J., Buzash-Pollert, E. & Studier, F. W. Proc. natn. Acad. Sci. U.S.A. 75, 2741–2745 (1978).
Shine, J. & Dalgarno, L. Nature 254, 34–38 (1975).
Hagenbüchle, O., Santer, M., Steitz, J. A. & Mans, R. J. Cell 13, 551–563 (1978).
Kozak, M. Cell 15, 1109–1123 (1978).
Barelle, F. E. & Brownlee, G. G. Nature 274, 84–87 (1978).
Both, G. W. FEBS Lett. 101, 220–224 (1979).
De Wachter, R. Nucleic Acids Res. 7, 2045–2054 (1979).
Sprague, K. U., Steitz, J. A., Grenley, R. M. & Stocking, C. E. Nature 267, 462–465 (1977).
Schwartz, Z. & Kössel, H. Nature 279, 520–522 (1979).
de Jonge, P., Klootwijk, J. & Planta, R. J. Nucleic Acids Res. 4, 3655–3663 (1977).
Alberty, H., Raba, M. & Gross, H. J. Nucleic Acids Res. 5, 425–434 (1978).
Hunt, J. A. Biochem. J. 120, 353–363 (1970).
Ziff, E. B. & Evans, R. M. Cell 15, 1463–1475 (1978).
Schroeder, Jr H. W., Liarakos, C. D., Gupta, R. C., Randerath, K. & O'Malley, B. W. Biochemistry 18, 5798–5808 (1979).
Pathak, M. A., Krämer, D. M. & Fitzpatrick, T. B. in Sunlight and Man—Normal and Abnormal Photobiologic Responses (eds Pathak, M. A. et al.) 335–387 (University of Tokyo Press, 1974).
Isaacs, S. T., Shen, C. J., Hearst, J. E. & Rapoport, H. Biochemistry 16, 1058–1064 (1977).
Nakashima, K., LaFiandra, A. J. & Shatkin, A. J. J. biol. Chem. 254, 8007–8014 (1979).
Both, G. W., Furuichi, Y., Muthukrishnan, S. & Shatkin, A. J. Cell 6, 185–195 (1975).
Muthukrishnan, S., Morgan, M., Banerjee, A. K. & Shatkin, A. J. Biochemistry 15, 5761–5768 (1976).
Kozak, M. & Shatkin, A. J. Meth. Enzym. 60, 360–375 (1979).
Kozak, M. & Shatkin, A. J. J. biol. Chem. 251, 4259–4266 (1976).
Kozak, M. & Shatkin, A. J. Cell 13, 201–212 (1978).
Marcus, A. J. biol. Chem. 245, 962–966 (1970).
Trachsel, H., Erni, B., Schreier, M. H. & Staehelin, T. J. molec. Biol. 116, 755–767 (1977).
Both, G. W., Furuichi, Y., Muthukrishnan, S. & Shatkin, A. J. J. molec. Biol. 104, 637–658 (1976).
England, T. E. & Uhlenbeck, O. C. Nature 275, 560–561 (1978).
Rosenberg, M. Nucleic Acids Res. 1, 653–671 (1974).
Furuichi, Y., Shatkin, A. J., Stavnezer, E. & Bishop, J. M. Nature 257, 618–620 (1975).
Kozak, M. Nature 269, 390–394 (1977).
Rabin, D. & Crothers, D. M. Nucleic Acids Res. 7, 689–703 (1979).
Donis-Keller, H., Maxam, A. M. & Gilbert, W. Nucleic Acids Res. 4, 2527–2538 (1977).
Eckhardt, H. & Lührmann, R. J. biol. Chem. 254, 11185–11188 (1979).
Taniguchi, T. & Weissmann, C. Nature 275, 770–772 (1978).
Roberts, T. M., Bikel, I., Yocum, R. R., Livingston, D. M. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 76, 5596–5600 (1979).
Azad, A. A. & Deacon, N. J. Biochem. biophys. Res. Commun. 86, 568–576 (1979).
Tsujimoto, Y. & Suzuki, Y. Cell 18, 591–600 (1979).
Legon, S. J. molec. Biol. 134, 219–240 (1979).
Rose, J. K. Cell 14, 345–353 (1978).
Rosenberg, M. & Paterson, B. M. Nature 279, 696–701 (1979).
Manning, R. F., Samols, D. R. & Gage, L. P. Gene 4, 153–166 (1978).
Furuichi, Y. & Shatkin, A. J. Proc. natn. Acad. Sci. U.S.A. 73, 3448–3452 (1976).
Nakashima, K. & Shatkin, A. J. J. biol. Chem. 253, 8680–8682 (1978).
Nakashima, K., Chanda, P. K., Deutsch, V., Banerjee, A. K. & Shatkin, A. J. J. Virol. 32, 838–844 (1979).
Pelham, H. R. B. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1976).
Darzynkiewicz, E. & Shatkin, A. J. Nucleic Acids Res. 8, 337–350 (1980).
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Nakashima, K., Darzynkiewicz, E. & Shatkin, A. Proximity of mRNA5′-region and 18S rRNA in eukaryotic initiation complexes. Nature 286, 226–230 (1980). https://doi.org/10.1038/286226a0
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DOI: https://doi.org/10.1038/286226a0
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