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Expression of cloned β-endorphin gene sequences by Escherichia coli

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Abstract

DNA coding for the opiate peptide β-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid β-galactosidase/β-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active β-endorphin.

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Authors and Affiliations

  1. Molecular Biology Unit, Research School of Biological Sciences, Australian National University, Canberra, Australia

    John Shine

  2. Howard Hughes Medical Institute Laboratories, Departments of Medicine and Biochemistry and Biophysics, and the Metabolic Research Unit, University of California, San Francisco, California, 94143

    Ivy Fettes, Nancy C. Y. Lan & John D. Baxter

  3. Department of Biochemistry, Columbia University, College of Physicians and Surgeons, New York, New York, 10032

    James L. Roberts

Authors
  1. John Shine
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  2. Ivy Fettes
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  3. Nancy C. Y. Lan
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  4. James L. Roberts
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  5. John D. Baxter
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Shine, J., Fettes, I., Lan, N. et al. Expression of cloned β-endorphin gene sequences by Escherichia coli. Nature 285, 456–461 (1980). https://doi.org/10.1038/285456a0

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  • DOI: https://doi.org/10.1038/285456a0

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