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Single ionic channels observed in tissue-cultured muscle

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Abstract

The extracellular patch clamp technique developed by Neher et al.1 to record the responses of single channels in skeletal muscle has provided firm evidence for the two-state nature of the conductance event in nicotinic endplate channels. We report here the use of the extracellular patch technique to record single-channel responses from tissue-cultured chick skeletal muscle cells. The temperature dependence of channel conductance and gating kinetics shows no evidence of discontinuous behaviour between 17 and 37 °C.

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, State University of New York at Buffalo, Buffalo, New York, 14214

    Deborah J. Nelson & Frederick Sachs

Authors
  1. Deborah J. Nelson
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  2. Frederick Sachs
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Nelson, D., Sachs, F. Single ionic channels observed in tissue-cultured muscle. Nature 282, 861–863 (1979). https://doi.org/10.1038/282861a0

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