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Three-dimensional structure of hyper-modified nucleoside Q located in the wobbling position of tRNA

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Abstract

The hyper-modified nucleoside Q (queuosine) is exclusively located in the wobbling position of anticodons of tRNATyr, tRNAHis, tRNAAsn and tRNAAsp that recognise codons NAcU (ref. 1). Queuosine and its hexose-containing derivatives2,3 are widely distributed in microorganisms, animals and plants4,5. We confirm here the chemical structure of queuosine as 7-(3, 4-trans-4, 5-cis-dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine2,6 (Fig. 1). The unique structural features of Q are the unusual cyclopentenediol side chain and the 7-deazaguanine ring. Queuosine is one of the most complicated modified nucleosides found thus far7.

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Authors and Affiliations

  1. Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo,

    Shigeyuki Yokoyama & Tatsuo Miyazawa

  2. Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113

    Yoichi Iitaka

  3. Biology Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, 104, Japan

    Ziro Yamaizumi, Hiroshi Kasai & Susumu Nishimura

Authors
  1. Shigeyuki Yokoyama
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  2. Tatsuo Miyazawa
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  3. Yoichi Iitaka
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  4. Ziro Yamaizumi
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  5. Hiroshi Kasai
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  6. Susumu Nishimura
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Yokoyama, S., Miyazawa, T., Iitaka, Y. et al. Three-dimensional structure of hyper-modified nucleoside Q located in the wobbling position of tRNA. Nature 282, 107–109 (1979). https://doi.org/10.1038/282107a0

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  • DOI: https://doi.org/10.1038/282107a0

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