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Isolation and characterisation of a yeast chromosomal replicator

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Abstract

A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.

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Authors and Affiliations

  1. Department of Biochemistry, Stanford University School of Medicine, Stanford, California, 94305

    D. T. Stinchcomb, K. Struhl & R. W. Davis

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  1. D. T. Stinchcomb
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  2. K. Struhl
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  3. R. W. Davis
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Stinchcomb, D., Struhl, K. & Davis, R. Isolation and characterisation of a yeast chromosomal replicator. Nature 282, 39–43 (1979). https://doi.org/10.1038/282039a0

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  • DOI: https://doi.org/10.1038/282039a0

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