Abstract
A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.
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References
Maaløe, O. & Kjeldgaard, N. O. Control of Macromolecular Synthesis (W. A. Benjamin, New York, 1966).
Hemstetter, C., Cooper, S., Diorucci, O. & Revelas, F. Cold Spring Harb. Symp. quant. Biol. 33, 809–822 (1968).
Carins, J. J. molec. Biol. 6, 208–213 (1963).
Jacob, E., Brenner, B. & Cuzin, F. Cold Spring Harb. Symp. quant. Biol. 28, 329–347 (1963).
Cold Spring Harb. Symp. quant. Biol. 43 (1978).
Hand, R. Cell 15, 317–325 (1978).
Sheinin, R. & Humbert, J. A. Rev. Biochem. 47, 277–316 (1978).
Pardee, A. B., Dubrow, R., Harmlin, J. L. & Kletzien, R. F. A. Rev. Biochem. 47, 715–750 (1978).
Struhl, K., Stinchcomb, D. T., Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 76, 1035–1039 (1979).
McDonnell, M. W., Simon, M. N. & Studier, F. W. J. molec. Biol. 110, 119–146 (1977).
Hinnen, A., Hicks, J. B. & Fink, G. R. Proc. natn. Acad. Sci. U.S.A. 75, 1929–1933 (1978).
Scherer, S. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. (in the press).
Hicks, J. B., Hinnen, A. & Fink, G. R. Cold Spring Harb. Symp. quant. Biol. 43, 1305–1313 (1978).
Wensink, P. C., Finnegan, D. J., Donelson, J. E. & Hogness, D. S. Cell 3, 315–325 (1974).
Hiraga, S. Proc. natn. Acad. Sci. U.S.A. 73, 198–202 (1976).
Yasuda, S. & Hirota, Y. Proc. natn. Acad. Sci. U.S.A. 74, 5458–5462 (1977).
Struhl, K. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 74, 5255–5259 (1977).
Struhl, K., Fink, G. R. & Davis, R. W. Nature 279, 78–79 (1979).
Struhl, K. & Davis, R. W. J. molec. Biol. (in the press).
Newlon, C. S., Petes, T. D., Hereford, L. M. & Fangman, W. K. Nature 247, 32–35 (1974).
Petes, T. D., Newlon, C. S., Byers, B. & Fangman, W. L. Cold Spring Harb. Symp. quant. Biol. 38, 9–16 (1973).
Petes, T. D. & Williamson, D. H. Expl Cell Res. 95, 103–110 (1975).
Burke, W. & Fangman, W. L. Cell 5, 263–269 (1975).
Hartwell, L. H. Bact. Rev. 38, 164–198 (1974).
Southern, E. J. molec. Biol. 98, 503–577 (1975).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
Cameron, J. R., Loh, E. Y. & Davis, R. W. Cell 16, 739–751 (1979).
Struhl, K., Cameron, J. R. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 73, 1471–1475 (1976).
Bolivar, R., et al. Gene 2, 95–113 (1977).
McClintock, B. Proc. natn. Acad. Sci. U.S.A. 18, 677–681 (1932), 25, 405–416 (1939).
Lis, J. T., Prestidge, L. & Hogness, D. S. Cell 14, 901–917 (1978).
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Stinchcomb, D., Struhl, K. & Davis, R. Isolation and characterisation of a yeast chromosomal replicator. Nature 282, 39–43 (1979). https://doi.org/10.1038/282039a0
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DOI: https://doi.org/10.1038/282039a0
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