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Two-step binding of eukaryotic ribosomes to brome mosaic virus RNA3

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Abstract

Although brome mosaic virus RNA3 has only one translatable cistron, it can bind two 80S ribosomes at initiation. One ribosome binds at the first AUG codon (bases 92–94). The other binds nearer the 5′ end at an entry or holding site. Disome formation is thus unrelated to a silent cistron ∼1,000 bases downstream.

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Authors and Affiliations

  1. Biophysics Laboratory of the Graduate School, and Biochemistry Department of the College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin, 53706

    Paul Ahlquist, Ranjit Dasgupta, Ding S. Shih, David Zimmern & Paul Kaesberg

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  1. Paul Ahlquist
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  2. Ranjit Dasgupta
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  3. Ding S. Shih
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  4. David Zimmern
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  5. Paul Kaesberg
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Ahlquist, P., Dasgupta, R., Shih, D. et al. Two-step binding of eukaryotic ribosomes to brome mosaic virus RNA3. Nature 281, 277–282 (1979). https://doi.org/10.1038/281277a0

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  • DOI: https://doi.org/10.1038/281277a0

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