Abstract
Ovalbumin is shown to contain an internal rather than an amino-terminal signal sequence. This internal signal can be recovered in a tryptic fragment which comprises residues 229–276 of mature ovalbumin and contains a region of striking sequence homology to amino-terminal cleaved signals of two other oviduct secretory proteins. The isolated tryptic fragment is used as a probe to demonstrate that a signal receptor is present in membrane vesicles derived from the rough but not in those derived from the smooth endoplasmic reticulum.
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Lingappa, V., Lingappa, J. & Blobel, G. Chicken ovalbumin contains an internal signal sequence. Nature 281, 117–121 (1979). https://doi.org/10.1038/281117a0
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DOI: https://doi.org/10.1038/281117a0
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