Skip to main content
SpringerLink
Log in

Rabbit β-globin mRNA production in mouse L cells transformed with cloned rabbit β-globin chromosomal DNA

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Mouse thymidine kinase-negative L cells were transformed with a cloned rabbit chromosomal β-globin gene linked to the cloned thymidine kinase gene of herpes simplex virus type 1. Most thymidine kinase-positive cell lines contained one or more copies of rabbit β-globin DNA and produced up to 2,000 copies of rabbit β-globin RNA per cell indistinguishable from its authentic counterpart. No mouse β-globin mRNA was detected.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Weissmann, C. Trends biochem. Sci. 3, N109–N111 (1978).

    CAS  Google Scholar 

  2. Mertz, J. E. & Gurdon, J. B. Proc. natn. Acad. Sci. U.S.A. 74, 1502–1506 (1977).

    Article  ADS  CAS  Google Scholar 

  3. Kressmann, A., Clarkson, S. G., Pirrotta, V. & Birnstiel, M. L. Proc. natn. Acad. Sci. U.S.A. 75, 1156–1180 (1978).

    Article  ADS  Google Scholar 

  4. Mueller, C., Graessmann, A. & Graessmann, M. Cell 15, 579–585 (1978).

    Article  CAS  Google Scholar 

  5. Ostro, M. J., Giacomoni, D., Lavelle, D., Paxton, W. & Dray, S. Nature 274, 921–923 (1978).

    Article  ADS  CAS  Google Scholar 

  6. Dimitriadis, G. J. Nature 274, 923–924 (1978).

    Article  ADS  CAS  Google Scholar 

  7. Rechsteiner, M. Natn. Cancer Inst. Monogr. 48, 57–64 (1978).

    Google Scholar 

  8. Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).

    Article  CAS  Google Scholar 

  9. Wigler, M. et al. Cell 11, 223–232 (1977).

    Article  CAS  Google Scholar 

  10. McCutchan, J. H. & Pagano, J. S. J. natn. Cancer Inst. 41, 351–357 (1968).

    CAS  Google Scholar 

  11. Lai, C.-J. & Nathans, D. Virology 60, 466–475 (1974).

    Article  CAS  Google Scholar 

  12. Mulligan, R. C., Howard, B. H. & Berg, P. Nature 277, 108–114 (1979).

    Article  ADS  CAS  Google Scholar 

  13. Szybalski, W., Szybalska, E. H. & Ragni, G. Natn. Cancer Inst. Monogr. 7, 75 (1962).

    Google Scholar 

  14. Boll, W. et al. Experientia 35, 957 (1979).

    Google Scholar 

  15. Weissmann, C. et al. 11th Miami Winter Symp., 1979 (in the press).

  16. van den Berg, J. et al. Nature 275, 37–44 (1978).

    Article  ADS  Google Scholar 

  17. Wigler, M., Pellicer, A., Silverstein, S. & Axel, R. Cell 14, 725–731 (1978).

    Article  CAS  Google Scholar 

  18. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  Google Scholar 

  19. Meyer, F., Heijneker, H., Weber, H. & Weissmann, C. Experientia 35, 972 (1979).

    Article  Google Scholar 

  20. Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).

    Article  CAS  Google Scholar 

  21. Weaver, R., Boll, W. & Weissmann, C. Experientia 35, 983 (1979).

    Google Scholar 

  22. Efstratiadis, A., Kafatos, F. C. & Maniatis, T. Cell 10, 571–585 (1977).

    Article  CAS  Google Scholar 

  23. Konkel, D. A., Tilghman, S. M. & Leder, P. Cell 15, 1125–1132 (1978).

    Article  CAS  Google Scholar 

  24. Parsons, J. T., Coffin, J. M., Haroz, R. K., Bromley, P. A. & Weissmann, C. J. Virol. 11, 761–774 (1973).

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Alwine, J. C., Kemp, D. H. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).

    Article  ADS  CAS  Google Scholar 

  26. Curtis, P. J., Mantei, N. & Weissmann, C. Cold Spring Harb. Symp. quant. Biol. 42, 971–984 (1977).

    Article  Google Scholar 

  27. Pellicer, A., Wigler, M., Axel, R. & Silverstein, S. Cell 14, 133–141 (1978).

    Article  CAS  Google Scholar 

  28. Ross, J. J. molec. Biol. 106, 403–420 (1976).

    Article  CAS  Google Scholar 

  29. Curtis, P. J. & Weissmann, C. J. molec. Biol. 106, 1061–1075 (1976).

    Article  CAS  Google Scholar 

  30. Kwan, S.-P., Wood, T. G. & Lingrel, J. B. Proc. natn. Acad. Sci. U.S.A. 74, 178–182 (1977).

    Article  ADS  CAS  Google Scholar 

  31. Bastos, R. N. & Aviv, H. Cell 11, 641–650 (1977).

    Article  CAS  Google Scholar 

  32. Curtis, P. J., Mantei, N., van den Berg, J. & Weissmann, C. Proc. natn. Acad. Sci. U.S.A. 74, 3184–3188 (1977).

    Article  ADS  CAS  Google Scholar 

  33. Kinniburgh, A. J., Mertz, J. E. & Ross, J. Cell 14, 681–693 (1978).

    Article  CAS  Google Scholar 

  34. Tilghman, S. M., Curtis, P. J., Tiemeier, D. C., Leder, P. & Weissmann, C. Proc. natn. Acad. Sci. U.S.A. 75, 1309–1313 (1978).

    Article  ADS  CAS  Google Scholar 

  35. Smith, K. & Lingrel, J. B. Nucleic Acids Res. 5, 3295–3301 (1978).

    Article  CAS  Google Scholar 

  36. Flavell, R. A., Grosveld, G. C., Grosveld, F. G., De Boer, E. & Kooter, J. M. 11th Miami Winter Symp., 1979 (in the press).

  37. De Robertis, E. M. & Mertz, J. E. Cell 12, 175–182 (1977).

    Article  CAS  Google Scholar 

  38. van den Berg, J., Beggs, J., van Ooyen, A. & Weissmann, C. Experientia 35, 982 (1979).

    Google Scholar 

  39. Ziff, E. B. & Evans, R. M. Cell 15, 1463–1475 (1978).

    Article  CAS  Google Scholar 

  40. Anderson, W. F. et al. Natn. Cancer Inst. Monog. 48, 65–73 (1978).

    Google Scholar 

  41. Wigler, M. et al. Cell 16, 777–785 (1979).

    Article  CAS  Google Scholar 

  42. Smith, H. O. & Birnstiel, M. L. Nucleic Acids Res. 3, 2387–2398 (1976).

    Article  CAS  Google Scholar 

  43. Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).

    Article  CAS  Google Scholar 

  44. Maniatis, T., Sim, G. K., Efstratiadis, A. & Kafatos, F. C. Cell 8, 163–182 (1976).

    Article  CAS  Google Scholar 

  45. Hofstetter, H., Schamböck, A., van den Berg, J. & Weissmann, C. Biochim. biophys. Acta 454, 587–591 (1976).

    Article  CAS  Google Scholar 

  46. Heyneker, H. L. et al. Nature 263, 748–752 (1976).

    Article  ADS  CAS  Google Scholar 

  47. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  Google Scholar 

  48. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  49. Wiegand, R. C., Godson, G. N. & Radding, C. M. J. biol. Chem. 250, 8848–8855 (1975).

    CAS  PubMed  Google Scholar 

  50. Sutcliffe, J. G. Nucleic Acids Res. 5, 2721–2728 (1978).

    Article  CAS  Google Scholar 

  51. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  52. McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).

    Article  ADS  CAS  Google Scholar 

  53. van Ooyen, A. et al. Science (in the press).

Download references

Author information

Authors and Affiliations

  1. Institut für Molekularbiologie I, Universität Zürich, 8093, Zürich, Switzerland

    Ned Mantei, Werner Boll & Charles Weissmann

Authors
  1. Ned Mantei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Werner Boll
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Charles Weissmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mantei, N., Boll, W. & Weissmann, C. Rabbit β-globin mRNA production in mouse L cells transformed with cloned rabbit β-globin chromosomal DNA. Nature 281, 40–46 (1979). https://doi.org/10.1038/281040a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/281040a0

  • Springer Nature Limited

This article is cited by

Search

Navigation