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Organisation and sequences at the 5′ end of a cloned complete ovalbumin gene

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Abstract

A clone which contains the complete chicken ovalbumin gene, including its leader coding sequences, has been isolated. From electron microscopic analysis of this DNA we conclude that the minimal size of the transcriptional unit for ovalbumin is 7.7 kilobases. The DNA sequence of the region surrounding the 5' end of the ovalbumin gene is presented. Comparison of this sequence with those of other eukaryotic genes reveals striking similarities, possibly related to a promoter region, approximately 30 base pairs upstream from the site coding for the 5′ end of the mRNA.

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Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de l'INSERM, Institut de Chimie Biologique, Faculté de Médicine, Strasbourg, France

    F. Gannon, K. O'Hare, F. Perrin, J. P. LePennec, C. Benoist, M. Cochet, R. Breathnach & P. Chambon

  2. Groupe de Biologie Moléculaire du Gène, ERA CNRS 201, Institut Pasteur, Paris, France

    A. Royal, A. Garapin & B. Cami

Authors
  1. F. Gannon
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  2. K. O'Hare
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  3. F. Perrin
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  4. J. P. LePennec
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  5. C. Benoist
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  6. M. Cochet
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  7. R. Breathnach
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  8. A. Royal
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  9. A. Garapin
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  10. B. Cami
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  11. P. Chambon
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Gannon, F., O'Hare, K., Perrin, F. et al. Organisation and sequences at the 5′ end of a cloned complete ovalbumin gene. Nature 278, 428–434 (1979). https://doi.org/10.1038/278428a0

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  • DOI: https://doi.org/10.1038/278428a0

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