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Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments

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Abstract

A 6.8-kilobase DNA fragment containing the sequence coding for the constant region of the mouse immunoglobulin γ1 heavy chain was cloned from total cellular DNA. Electron microscopic and nucleotide sequencing studies showed that the three protein domains and the hinge region are encoded in separate DNA segments.

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References

  1. Tonegawa, S., Maxam, A. M., Tizard, R., Bernard, O. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 3518–3522 (1978).

    Google Scholar 

  2. Glover, D. M. & Hogness, D. S. Cell 10, 167–176 (1977).

    CAS  PubMed  Google Scholar 

  3. Wellauer, P. K. & Dawid, I. B. Cell 10, 193–212 (1977).

    CAS  PubMed  Google Scholar 

  4. Berget, S. M., Moore, C. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 74, 3171–3175 (1977).

    ADS  CAS  Google Scholar 

  5. Chow, L. T., Gelinas, R. E., Brocker, T. R. & Roberts, R. J. Cell 12, 1–8 (1977).

    CAS  PubMed  Google Scholar 

  6. Brack, C. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 74, 5652–5656 (1977).

    ADS  CAS  Google Scholar 

  7. Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).

    CAS  PubMed  Google Scholar 

  8. Tilghman, S. M. et al. Proc. natn. Acad. Sci. U.S.A. 75, 725–729 (1978).

    ADS  CAS  Google Scholar 

  9. Breathnach, R., Mandel, J. L. & Chambon, P. Nature 270, 314–319 (1977).

    ADS  CAS  PubMed  Google Scholar 

  10. Doel, M. T., Houghton, M., Cook, E. A. & Garey, N. H. Nucleic Acids Res. 4, 3701–3713 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Goodman, H. M., Olson, M. V. & Hall, B. D. Proc. natn. Acad. Sci. U.S.A. 74, 5453–5457 (1977).

    ADS  CAS  Google Scholar 

  12. Valenzuella, P., Venegas, A., Weinberg, F., Bishop, R. & Rutter, W. J. Proc. natn. Acad. Sci. U.S.A. 75, 190–194 (1978).

    ADS  Google Scholar 

  13. Hill, R. L., Delaney, R., Fellows, R. E. & Lebovitz, H. E. Proc. natn. Acad. Sci. U.S.A. 56, 1762–1768 (1966).

    ADS  CAS  Google Scholar 

  14. Singer, S. J. & Doolittle, R. F. Science 153, 13–17 (1966).

    ADS  CAS  PubMed  Google Scholar 

  15. Edelman, G. M. et al. Proc. natn. Acad. Sci. U.S.A. 63, 78–85 (1969).

    ADS  CAS  Google Scholar 

  16. Poljak, R. et al. Proc. natn. Acad. Sci. U.S.A. 70, 3305–3310 (1973).

    ADS  CAS  Google Scholar 

  17. Padlan, E. A. et al. Nature new Biol. 245, 165–167 (1973).

    CAS  PubMed  Google Scholar 

  18. Brack, C., Hirama, M., Lenhard-Schuller, R. & Tonegawa, S. Cell 15, 1–14 (1978).

    CAS  PubMed  Google Scholar 

  19. Lenhard-Schuller, R., Hohn, B., Brack, C., Hirama, M. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 75, 4709–4713 (1978).

    ADS  CAS  Google Scholar 

  20. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    CAS  PubMed  Google Scholar 

  21. Rogers, J. H., Clark, P. & Salser, W. (submitted).

  22. Tiemeier, D. C., Enquist, L. & Leder, P. Nature 263, 526–528 (1976).

    ADS  CAS  Google Scholar 

  23. Hohn, B. & Murray, K. Proc. natn. Acad. Sci. U.S.A. 74, 3259–3263 (1977).

    ADS  CAS  Google Scholar 

  24. Benton, W. D. & Davis, R. W. Science 196, 180–192 (1977).

    ADS  CAS  PubMed  Google Scholar 

  25. White, R. L. & Hogness, D. S. Cell 10, 177–182 (1977).

    CAS  PubMed  Google Scholar 

  26. Adetugbo, K. J. biol. Chem. 253, 6068–6075 (1978).

    CAS  PubMed  Google Scholar 

  27. Tilghman, S. M., Curtis, P. J., Tiemeier, D. C., Leder, P. & Weissmann, C. Proc. natn. Acad. Sci. U.S.A. 75, 1309–1313 (1978).

    ADS  CAS  Google Scholar 

  28. Knapp, G., Beckmann, J., Johnson, P. F., Fuhrman, S. A. & Abelson, J. Cell 14, 221–236 (1978).

    CAS  PubMed  Google Scholar 

  29. O'Farrell, P. Z., Cordell, B., Valenzuela, P., Rutter, W. J. & Goodman, H. M. Nature 274, 438–445 (1978).

    ADS  CAS  PubMed  Google Scholar 

  30. Bernard, O., Hozumi, N. & Tonegawa, S. Cell 15, 1133–1144 (1978).

    CAS  PubMed  Google Scholar 

  31. Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

    ADS  CAS  Google Scholar 

  32. Reddy, V. B. et al. Science 200, 494–502 (1978).

    ADS  CAS  PubMed  Google Scholar 

  33. Van den Berg, J. et al. Nature 276, 37–44 (1978).

    ADS  CAS  PubMed  Google Scholar 

  34. Catterall, J. F. et al. Nature 275, 510–514 (1978).

    ADS  CAS  PubMed  Google Scholar 

  35. Franklin, E. C. & Contemp. Topics Molec. Immun. 4, 89–126 (1975).

    CAS  Google Scholar 

  36. Adetugbo, K., Milstein, C. & Becker, D. S. Nature 265, 299–304 (1977).

    ADS  CAS  PubMed  Google Scholar 

  37. Kuehl, W. M. & Sharff, M. D. J. molec. Biol. 89, 409–421 (1974).

    CAS  PubMed  Google Scholar 

  38. Burstein, Y. & Schechter, I. Biochemistry 17, 2392–2400 (1978).

    CAS  PubMed  Google Scholar 

  39. Rose, S. M., Kuehl, M. & Smith, G. P. Cell 12, 453–462 (1977).

    CAS  PubMed  Google Scholar 

  40. Davis, R. W., Simon, M. & Davidson, N. Meth. Enzym. 21D, 413–428 (1971).

    Google Scholar 

  41. Westmorland, B. C., Szybalski, W. & Ris, W. Science 163, 1343–1346 (1969).

    ADS  Google Scholar 

  42. Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    ADS  CAS  Google Scholar 

  43. Sanger, F. & Coulson, A. R. FEBS Lett. 87, 107–116 (1978).

    CAS  PubMed  Google Scholar 

  44. Bennich, H. & von Bahr Lindsm, H. in Progress in Immunology II, Vol. 1 (eds Brent, L. & Holborow, J.) 49–58 (North-Holland, Amsterdam, 1974).

    Google Scholar 

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Authors and Affiliations

  1. Basel Institute for Immunology, 487 Grenzacherstrasse, Postfach, CH-4005, Basel, Switzerland

    Hitoshi Sakano, Konrad Hüppi, Christine Brack, André Traunecker, Richard Maki & Susumu Tonegawa

  2. Molecular Biology Institute and Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles, California, 90024

    John H. Rogers & Randolph Wall

Authors
  1. Hitoshi Sakano
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  2. John H. Rogers
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  3. Konrad Hüppi
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  4. Christine Brack
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  5. André Traunecker
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  6. Richard Maki
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  7. Randolph Wall
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  8. Susumu Tonegawa
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Sakano, H., Rogers, J., Hüppi, K. et al. Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments. Nature 277, 627–633 (1979). https://doi.org/10.1038/277627a0

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  • DOI: https://doi.org/10.1038/277627a0

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