Skip to main content
SpringerLink
Log in

Induction of specific antibodies to poly(ADP–ribose) in rabbits by double-stranded RNA, poly(A)·poly(U)

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

WE have reported1 the presence of naturally occurring antibodies to poly(ADP–ribose) in the sera of patients with systemic lupus erythematosus (SLE). During our study we discovered a population of antibodies cross-reacting with poly(A)·poly(U) among the antibodies to poly(ADP–ribose) and found that this population could be completely removed by absorption of the SLE sera with poly(A)·poly(U)1. The mechanism of the cross-reaction to poly(A)·poly(U) of some antibodies to poly(ADP–ribose) is unknown. Poly(ADP–ribose) is a biopolymer which is synthesised by nuclear enzyme(s)2. Recent advance in the study of this biopolymer has been reviewed3,4. We have found5 that poly(ADP–ribose) shows strong antigenicity in rabbits and that rabbit antibodies to poly(ADP–ribose) do not cross-react at all with synthetic homopolynucleotides. However, the cross-reactions of rabbit antibodies with double-stranded RNA, poly(A)·poly(U) or poly(I)·poly(C), have not been fully elucidated. Therefore, we tested whether poly(A)·poly(U) can induce specific antibodies to poly(ADP–ribose) and, conversely, whether poly(ADP–ribose) can induce antibodies cross-reacting with poly(A)·poly(U). We describe here that induction of specific antibodies to poly(ADP–ribose) in rabbits could be achieved by injecting a complex of poly(A)·poly(U) and methylated bovine serum albumin (MBSA) in Freund's complete adjuvant (FCA); moreover, poly(A)·poly(U) has a specific effect in inducing antibodies to poly(ADP–ribose), as neither poly(I)·poly(C) nor poly(A) induce antibodies to poly(ADP–ribose).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kanai, Y. et al. Nature 265, 175–177 (1977).

    Article  ADS  CAS  Google Scholar 

  2. Sugimura, T. Progr. Nucleic Acid Res. molec. Biol. 13, 125–151 (1973).

    Google Scholar 

  3. Hilz, H. & Stone, P. Rev. physiol. biochem. Pharmac. 76, 1–58 (1976).

    Article  CAS  Google Scholar 

  4. Hayaishi, O. & Ueda, K. A. Rev. Biochem. 46, 95–116 (1977).

    Article  CAS  Google Scholar 

  5. Kanai, Y., Miwa, M., Matsushima, T. & Sugimura, T. Biochem. biophys. Res. Commun. 59, 300–306 (1974).

    Article  CAS  Google Scholar 

  6. Sugimura, T., Yoshimura, N., Miwa, M., Nagai, H. & Nagao, M. Archs Biochem. Biophys. 147, 660–665 (1971).

    Article  CAS  Google Scholar 

  7. Kanai, Y., Miwa, M., Matsushima, T. & Sugimura, T. Immunology 34, 501–508 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Nisonoff, A., Wissler, F. C., Lipman, L. N. & Woernly, D. L. Archs Biochem. Biophys. 89, 230–244 (1960).

    Article  CAS  Google Scholar 

  9. Mandy, W. J. & Nisonoff, A. J. biol. Chem. 238, 206–213 (1963).

    Google Scholar 

  10. Braun, W. & Nakano, N. Science 157, 819–821 (1967).

    Article  ADS  CAS  Google Scholar 

  11. Schmidtke, J. R. & Johnson, A. G. J. Immun. 106, 1191–1200 (1971).

    CAS  PubMed  Google Scholar 

  12. Cone, R. E. & Marchalonis, J. J. Aust. J. exp. biol. med. Sci. 50, 69–77 (1972).

    Article  CAS  Google Scholar 

  13. Brawn, W. Nature 224, 1024–1025 (1969).

    Article  ADS  Google Scholar 

  14. Truner, W., Chan, S. P. & Chirigos, M. A. Proc. Soc. exp. Biol. 133, 334–338 (1970).

    Article  Google Scholar 

  15. Mäkelä, O. J. Immun. 95, 378–386 (1965).

    PubMed  Google Scholar 

  16. Walters, C. S. & Wigzell, H. Cell Immun., 5, 570–578 (1972).

    Article  CAS  Google Scholar 

  17. Merchant, B. & Inman, J. K. J. exp. Med. 145, 372–389 (1977).

    Article  CAS  Google Scholar 

  18. Fedoroff, N., Wellauer, P. K. & Wall, R. Cell 10, 597–610 (1977).

    Article  CAS  Google Scholar 

  19. Györkey, F., Sinkovics, J. G., Min, K. W. & Györkey, P. Am. J. Med. 53, 148–158 (1972).

    Article  Google Scholar 

  20. Weissman, C. & Ochoa, S. Progr. Nucleic Acid Res. molec. Biol. 6, 353–399 (1967).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, 108, Japan

    YOSHIYUKI KANAI & TAIJIRO MATSUSHIMA

  2. Biochemistry Division, National Cancer Center Research Institute, Chuo-ku, Tokyo, 104

    TAKASHI SUGIMURA

Authors
  1. YOSHIYUKI KANAI
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. TAIJIRO MATSUSHIMA
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. TAKASHI SUGIMURA
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

KANAI, Y., MATSUSHIMA, T. & SUGIMURA, T. Induction of specific antibodies to poly(ADP–ribose) in rabbits by double-stranded RNA, poly(A)·poly(U). Nature 274, 809–812 (1978). https://doi.org/10.1038/274809a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/274809a0

  • Springer Nature Limited

This article is cited by

Search

Navigation