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Differential metabolism and leakage of protein in an inherited cataract and a normal lens cultured with ouabain

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Abstract

Ocular lenses in Nakano mice showed marked changes in synthesis, degradation and leakage of protein during cataractogenesis. The cataract-associated changes included the differential lowering of crystallin synthesis, the cleavage of crystallin polypeptides to lower molecular weight forms and the leakage of crystallins from cultured lenses. Ouabain treatment of normal lenses induced these alterations, suggesting that changes in the intracellular levels of Na+ and K+ affect the anabolism and catabolism of protein during cataract formation.

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Authors and Affiliations

  1. Section on Cellular Differentiation, Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20014

    Joram Piatigorsky

  2. Section on Biochemistry, Laboratory of Vision Research, National Eye Institute, National Institutes of Health, Bethesda, Maryland, 20014

    Henry N. Fukui & Jin H. Kinoshita

Authors
  1. Joram Piatigorsky
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  2. Henry N. Fukui
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  3. Jin H. Kinoshita
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Piatigorsky, J., Fukui, H. & Kinoshita, J. Differential metabolism and leakage of protein in an inherited cataract and a normal lens cultured with ouabain. Nature 274, 558–562 (1978). https://doi.org/10.1038/274558a0

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  • DOI: https://doi.org/10.1038/274558a0

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