Abstract
THE major histocompatibility complex (MHC) controls the mixed lymphocyte reaction (MLR) by a dual mechanism involving both the I-region and the K/D-loci products (for a review, see ref. 1). Most information about the MLR has so far been obtained by genetic studies using congenic resistant mouse strains that differ only at defined MHC loci. To dissect the events of the MLR further, it would be advantageous to use purified MHC antigens to gain control not only over the genetical but also the physico-chemical nature of the stimulating antigens. We describe here how in a first attempt to use highly purified MHC antigens as stimulatory agents in the MLR, we have succeeded in anchoring the antigens to liposomes in such a way that they give rise to a good response in the secondary MLR.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Möller, G. (ed.) Transplantation Rev. 12, 3–140 (1972).
Hayman, M. J. & Crumpton, M. J. Biochem. biophys. Res. Commun. 47, 923–930 (1972).
Snary, D., Goodfellow, P., Bodmer, W. F. & Crumpton, M. J. Nature 258, 240–242 (1975).
Laemmli, U. K. Nature 227, 680–685 (1970).
Cantor, H. & Boyse, E. A. J. exp. Med. 141, 1376–1389 (1975).
Cantor, H. & Boyse, E. A. J. exp. Med. 141, 1390–1399 (1975).
Alter, B. J. et al. J. exp. Med. 143, 1005–1014 (1976).
Andersson, L. C. & Häyry, P. Eur. J. Immun. 3, 595–599 (1973).
Peterson, P. A. et al. Cold Spring Harb. Symp. quant. Biol. 41, 331–339 (1977).
Hunter, W. M. & Greenwood, F. C. Nature 194, 495–496 (1962).
Peterson, P. A., Rask, L. & Lindblom, J. B. Proc. natn. Acad. Sci. U.S.A. 71, 35–39 (1974).
Kvist, S., Sandberg-Trägårdh, L., Östberg, L. & Peterson, P. A. Biochemistry 16, 4415–4422 (1977).
Kvist, S., Östberg, L. & Peterson, P. A. Scand. J. Immun. (in the press).
Östberg, L., Sege, K., Rask, L. & Peterson, P. A. Folia Biol. Praha 22, 372–373 (1976).
Klareskog, L., Trägårdh, L., Lindblom, J. B. & Peterson, P. A. Scand. J. Immun. (in the press).
Klareskog, L. et al. Nature 265, 248–251 (1977).
Cullen, S. E., Freed, J. H. & Nathenson, S. G. Transplantation Rev. 30, 236–270 (1976).
Brunner, J., Skrabal, P. & Hauser, H. Biochim. biophys. Acta 455, 322–331 (1976).
Chen, P. S., Toribara, T. Y. & Warner, H. Analyt. Chem. 28, 1756–1759 (1956).
Julius, M. H., Simpson, E. & Herzenberg, L. A. Eur. J. Immun. 3, 645–649 (1973).
Miller, R. G. & Phillips, R. A. J. cell. Physiol. 73, 191–202 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
CURMAN, B., ÖSTBERG, L. & PETERSON, P. Incorporation of murine MHC antigens into liposomes and their effect in the secondary mixed lymphocyte reaction. Nature 272, 545–547 (1978). https://doi.org/10.1038/272545a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/272545a0
- Springer Nature Limited
This article is cited by
-
Induction of regulatory T-lymphocyte responses by liposomes carrying major histocompatibility complex molecules and foreign antigen
Nature (1985)
-
A membrane-bound fluorescent probe to detect phospholipid vesicle-cell fusion
The Journal of Membrane Biology (1980)
-
The partial isolation of subcellular MHC products which are recognized by alloimmune T lymphocytes
Immunogenetics (1980)
-
Bilayer lipid membranes (BLM): Study of antigen-antibody interactions
Journal of Bioenergetics and Biomembranes (1978)