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Assessment of specificity of oestrogen receptor-DNA interaction by a competitive assay

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Abstract

ALTHOUGH hormonal regulation of gene expression and the nature of specific interactions between steroid hormone receptor and genome have attracted growing interest1–5, very little is known about the molecular nature of receptor–DNA interaction4–6. As a first step in unravelling the specificity and the dynamics of the interaction between uterine oestrogen receptor and DNA7–11, we have selected a well defined model system: a synthetic DNA and DNA–cellulose competition assay. We have found that the AT-rich segment of the double stranded DNA in its intact conformation is required for optimum receptor binding. We have examined oestrogen receptor binding to synthetic DNA with well defined sequences, and determined whether the receptor favours double-stranded or single-stranded regions of the DNA (unwinding protein generally binds preferentially to single-stranded DNA12). Finally, we have studied the effect of an intercalating drug, actinomycin D, on the receptor binding.

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Authors and Affiliations

  1. Research Institute of the Hospital for Joint Diseases,

    JOHN KALLOS & VINCENT P. HOLLANDER

  2. Medical Center, Mount Sinai School of Medicine, 1919 Madison Avenue, New York, New York, 10035

    JOHN KALLOS & VINCENT P. HOLLANDER

Authors
  1. JOHN KALLOS
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  2. VINCENT P. HOLLANDER
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KALLOS, J., HOLLANDER, V. Assessment of specificity of oestrogen receptor-DNA interaction by a competitive assay. Nature 272, 177–179 (1978). https://doi.org/10.1038/272177a0

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  • DOI: https://doi.org/10.1038/272177a0

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