Skip to main content
SpringerLink
Log in

Nucleotide sequence of the origin of replication in bacteriophage ΦX174 RF DNA

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

The gene A protein of bacteriophage ΦX174 has been used in vitro to convert ΦX RFI DNA into the relaxed RFII form by nicking the viral strand. The nucleotide sequence at the 3′ end of the nick has been determined as – – T G C T C C C C C A A C T T GOH. This sequence gives the exact position of the origin of ΦX RF DNA replication.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gilbert, W. & Dressler, D. H. Cold Spring Harb. Symp. quant. Biol. 33, 473–484 (1968).

    Article  CAS  PubMed  Google Scholar 

  2. Schröder, C. H., Erben, E. & Kaerner, H. J. molec. Biol. 79, 599–613 (1973).

    Article  PubMed  Google Scholar 

  3. Eisenberg, S. & Denhardt, D. T. Proc. natn. Acad. Sci. U.S.A. 71, 984–988 (1974).

    Article  ADS  CAS  Google Scholar 

  4. Baas, P. D. & Jansz, H. S. J. molec. Biol. 63, 569–576 (1972).

    Article  CAS  PubMed  Google Scholar 

  5. Baas, P. D., Jansz, H. S. & Sinsheimer, R. L. J. molec. Biol. 102, 633–656 (1976).

    Article  CAS  PubMed  Google Scholar 

  6. Johnson, P. H. & Sinsheimer, R. L. J. molec. Biol. 83, 47–61 (1974).

    Article  CAS  PubMed  Google Scholar 

  7. Godson, G. N. J. molec. Biol. 90, 127–141 (1974).

    Article  CAS  PubMed  Google Scholar 

  8. Eisenberg, S., Harbers, B., Hours, C. & Denhardt, D. T. J. molec. Biol. 99, 107–123 (1975).

    Article  CAS  PubMed  Google Scholar 

  9. Tessman, E. S. J. molec. Biol. 17, 218–236 (1966).

    Article  CAS  PubMed  Google Scholar 

  10. Francke, B. & Ray, D. S. J. molec. Biol. 61, 565–586 (1971).

    Article  CAS  PubMed  Google Scholar 

  11. Francke, B. & Ray, D. S. Proc. natn. Acad. Sci. U.S.A. 69, 475–479 (1972).

    Article  ADS  CAS  Google Scholar 

  12. Henry, T. J. & Knippers, R. Proc. natn. Acad. Sci. U.S.A. 71, 1549–1553 (1974).

    Article  ADS  CAS  Google Scholar 

  13. Eisenberg, S., Scott, J. F. & Kornberg, A. Proc. natn. Acad. Sci. U.S.A. 73, 1594–1597 (1976).

    Article  ADS  CAS  Google Scholar 

  14. Ikeda, J., Yudelevich, A. and Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 73, 2669–2673 (1976).

    Article  ADS  CAS  Google Scholar 

  15. Jacob, F., Brenner, S. & Cuzin, F. Cold Spring Harb. Symp. quant. Biol. 28, 329–347 (1963).

    Article  CAS  Google Scholar 

  16. Sanger, F. et al. Nature 265, 687–695 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Linney, E. & Hayashi, M. Nature new Biol. 245, 6–8 (1973).

    Article  CAS  PubMed  Google Scholar 

  18. Sharp, P. A., Sugden, B. & Sambrook, J. Biochemistry 12, 3055–3063 (1973).

    Article  CAS  PubMed  Google Scholar 

  19. Keller, W. & Wendel, I. Cold Spring Harb. Symp. quant. Biol. 39, 199–208 (1974).

    Article  Google Scholar 

  20. Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 101–116 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).

    Article  ADS  CAS  Google Scholar 

  22. Maniatis, T., Jeffrey, A. & van de Sande, H. Biochemistry 14, 3787–3794 (1975).

    Article  CAS  PubMed  Google Scholar 

  23. Brownlee, G. G. & Sanger, F. Eur. J. Biochem. 21, 395–399 (1969).

    Article  Google Scholar 

  24. Galibert, F., Sedat, J. & Ziff, E. J. J. molec. Biol. 87, 377–407 (1974).

    Article  CAS  PubMed  Google Scholar 

  25. Tu, C. D., Jay, E., Bahl, C. P. & Wu, R. Analyt. Biochem. 74, 73–93 (1976).

    Article  CAS  PubMed  Google Scholar 

  26. Weisbeek, P. J., Vereijken, J. M., Baas, P. D., Jansz, H. S. & Van Arkel, G. A. Virology 72, 61–71 (1976).

    Article  CAS  PubMed  Google Scholar 

  27. Subramanian, K. M., Dhar, R. & Weissman, S. M. J. biol. Chem. 252, 355–367 (1977).

    CAS  PubMed  Google Scholar 

  28. Soeda, E., Miura, K., Nakaso, A. & Kimura, G. FEBS Lett. 79, 383–388 (1977).

    Article  CAS  PubMed  Google Scholar 

  29. Tomizawa, J., Ohmori, H. & Bird, R. E. Proc. natn. Sci. Acad. U.S.A. 74, 1865–1869 (1977).

    Article  ADS  CAS  Google Scholar 

  30. Schaller, H., Uhlmann, A. & Geider, K. Proc. natn. Acad. Sci. U.S.A. 73, 49–53 (1976).

    Article  ADS  CAS  Google Scholar 

  31. Rowen, S. L. & Bouché, J. P. Fedn. Proc. 35, Abstr. 305 (1976).

    Google Scholar 

  32. Marians, K. J., Ikeda, J., Schlagman, S. & Hurwitz, J. Proc. natn. Acad. Sci. U.S.A. 74, 1965–1968 (1977).

    Article  ADS  CAS  Google Scholar 

  33. Jacob, R. J., Lebowitz, J. & Kleinschmidt, A. K. J. Virol. 13, 1176–1185 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  34. Robinson, A. J. & Bellet, A. J. D. Cold Spring Harb. Symp. quant. Biol. 39, 523–531 (1974).

    Article  Google Scholar 

  35. Kasamatsu, H. & Wu, M. Proc. natn. Acad. Sci. U.S.A. 73, 1945–1949 (1976).

    Article  ADS  CAS  Google Scholar 

  36. Laemmli, U. K. Nature, 227, 680–685 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  37. Denhardt, D. T. CRC Crit. Rev. Microbiol. 4, 161–222 (1975).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Cell Biology, State University of Utrecht, Utrecht, The Netherlands

    S. A. Langeveld, G. A. van Arkel & P. J. Weisbeek

  2. Institute of Molecular Biology, State University of Utrecht, Utrecht, The Netherlands

    S. A. Langeveld, A. D. M. van Mansfeld, P. D. Baas, H. S. Jansz, G. A. van Arkel & P. J. Weisbeek

  3. Laboratory of Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands

    P. D. Baas & H. S. Jansz

Authors
  1. S. A. Langeveld
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. D. M. van Mansfeld
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. D. Baas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. S. Jansz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. A. van Arkel
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. J. Weisbeek
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Langeveld, S., van Mansfeld, A., Baas, P. et al. Nucleotide sequence of the origin of replication in bacteriophage ΦX174 RF DNA. Nature 271, 417–420 (1978). https://doi.org/10.1038/271417a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/271417a0

  • Springer Nature Limited

This article is cited by

Search

Navigation