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Electrophoretic mobility as a sensitive probe of lectin–lymphocyte interaction

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Abstract

TREATMENT of murine T lymphocytes with concanavalin A (con A) at concentrations as low as 10−17 g ml−1 produces significant increases in their electrophoretic mobility. The effect is blocked by α-methyl-D-mannopyranoside (α-MM). Phytohaemagglutinin (PHA) produces similar changes in T-cell mobility while treatment with lipopolysaccharide (LPS), a B-lymphocyte mitogen, does not. We present here evidence indicating that the altered mobility of con A-treated T cells is due to the rapid release of a factor from the few cells reacting directly with the lectin.

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Authors and Affiliations

  1. Department of Pathology, Good Samaritan Medical Center, Portland, Oregon, 97210

    PHILIP BLUME

  2. Immune Disease Department, Oregon Regional Primate Research Center, Beaverton, Oregon, 97005

    ARTHUR MALLEY

  3. Department of Neurology, University of Oregon Health Sciences Center, Portland, Oregon, 97201

    ROBERT J. KNOX & GEOFFREY V. F. SEAMAN

Authors
  1. PHILIP BLUME
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  2. ARTHUR MALLEY
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  3. ROBERT J. KNOX
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  4. GEOFFREY V. F. SEAMAN
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BLUME, P., MALLEY, A., KNOX, R. et al. Electrophoretic mobility as a sensitive probe of lectin–lymphocyte interaction. Nature 271, 378–380 (1978). https://doi.org/10.1038/271378a0

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