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Cell surface changes in alloantigen activated T lymphocytes

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Abstract

MANY interactions which take place between T lymphocytes and other cells are presumably mediated at the level of the cell surface. In a mixed leukocyte culture (MLC), interaction between allogeneic lymphocytes results in transformation of the responding small T lymphocytes to large blast-like cells and their development into killer, helper1 and suppressor lymphocytes2. Some blasts subsequently revert to small memory or ‘secondary-type’ lymphocytes3. An important question is whether significant changes occur in T lymphocyte membrane proteins as a result of these interactions. We have attempted to answer this question by comparing iodine-labelled surface proteins of the following cells: T lymphocytes before stimulation, blast cells and small lymphocytes purified by lg velocity sedimentation from an MLC on day five, and reversion T lymphocytes following an additional 5 d of culture of the separated blast T lymphocytes. The results demonstrate that iodine-labelled components change during blast transformation and provide evidence for membrane differences between ‘secondary-type’ T lymphocytes and unsensitised cells.

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Authors and Affiliations

  1. Immunobiology Research Center and Departments of Medical Genetics, Surgery and Pediatrics, The University of Wisconsin, Madison, Wisconsin, 53706

    BRIAN DUNLAP, FRITZ H. BACH & MARILYN L. BACH

Authors
  1. BRIAN DUNLAP
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  2. FRITZ H. BACH
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  3. MARILYN L. BACH
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DUNLAP, B., BACH, F. & BACH, M. Cell surface changes in alloantigen activated T lymphocytes. Nature 271, 253–255 (1978). https://doi.org/10.1038/271253a0

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