Abstract
METHODS for sequencing nucleic acids have improved significantly in the last few years due to the development of rapid ‘read-off’ gel methods. The first method to be developed was the ‘plus and minus’ copying method for DNA1 and an analogous copying method for RNA now exists2. A different and more direct method for sequencing DNA has been developed3, but no direct method is available for RNA. We have developed such a method and here we describe its use in establishing the sequence of tyrosine tRNA of Bacillus stearothermophilus. This sequence is especially interesting as the tyrosine tRNA and its specific synthetase in B. stearothermophilus offer an attractive experimental system for studying the specific interaction of tRNA and synthetases, which is now possible because of progress in studying the three-dimensional structure of the protein by X-ray crystallography4.
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SIMONCSITS, A., BROWNLEE, G., BROWN, R. et al. New rapid gel sequencing method for RNA. Nature 269, 833–836 (1977). https://doi.org/10.1038/269833a0
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DOI: https://doi.org/10.1038/269833a0
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