Abstract
PROTEIN synthesis in cell-free systems from mouse L-cells pretreated with the antiviral agent interferon1 shows an enhanced sensitivity to inhibition by double-stranded RNA (dsRNA) (refs 2–4). A dsRNA-dependent protein kinase5–7 and a heat-stable, low-molecular-weight inhibitor (LMW inhibitor) of protein synthesis which is formed in such systems on incubation with ATP and dsRNA (ref. 7), seem likely to be involved. We have shown8 that the LMW inhibitor can be conveniently synthesised using a highly-purified enzyme fraction from interferon-treated cells bound to dsRNA on an inert support. Here we report its partial characterisation.
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KERR, I., BROWN, R. & HOVANESSIAN, A. Nature of inhibitor of cell-free protein synthesis formed in response to interferon and double-stranded RNA. Nature 268, 540–542 (1977). https://doi.org/10.1038/268540a0
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DOI: https://doi.org/10.1038/268540a0
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