Abstract
PURIFIED liver lysosomal glycosidases display very short plasma survival times following intravenous injection. The mechanism by which these hydrolases are cleared from plasma involves specific recognition of the enzymes by way of sites associated with the enzyme molecules. In our study, a modified plasma glycoprotein terminating in N-acetyl-glucosamine has been shown to inhibit recognition and clearance of several purified lysosomal glycosidases.
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Touster, O., Molec. cell. Biochem., 2, 169–177 (1973).
Bishayee, S., and Bachhawat, B. K., Biochim. biophys. Acta, 334, 378–388 (1974).
deDuve, C., Pressman, B., Gianetto, R., Wattiaux, R., and Applemans, F., Biochem. J., 60, 604–617 (1955).
Brady, R. O., Pentchey, P., and Gal, A. E., Fedn. Proc., 34, 1310–1315 (1975).
Achord, D. T., Frankel, H., Glaser, J., Brot, F., and Sly, W. S., Proc. 27th A. Mtg Amer. Soc. hum. Genet., 10A (1975).
Stahl, P., Mandell, B., Rodman, J., Schlesinger, P., and Lang, S., Archs Biochem. Biophys., 170, 536–546 (1975).
Stahl, P., Rodman, J. S., and Schlesinger, P., Archs Biochem. Biophys. (in the press).
Schlesinger, P., Miller, J., and Stahl ., Proc. Am. Soc. Cell Biol. (1976). (Abstract).
Ashwell, G., and Morell, A., in Advances in Enzymology (edit. by Meister, A.), 41, 99–128 (John Wiley and Sons, New York, 1974).
Morell, A., Gregoriadis, G., Scheinberg, I., Hickman, J., and Ashwell, G., J. biol. Chem., 246, 1461–1467 (1971).
Kawasaki, T., and Ashwell, G., J. biol. Chem., 251, 1296–1302 (1976).
Stockert, R., Morell, A., and Scheinberg, I., Biochem. biophys. Res. Commun., 68, 988–993 (1976).
Lunney, J., and Ashwell, G., Proc natn. Acad. Sci. U.S.A., 73, 341–343 (1976).
Stahl, P., Six, H., Rodman, J. S., Schlesinger, P., Tulsiani, D., and Touster, O., Proc. natn. Acad. Sci. U.S.A. (in the press).
Stahl, P., and Touster, O., J. biol. Chem., 246, 5398–5406 (1971).
Keller, R. K., and Touster, O., J. biol. Chem., 250, 4765–4769 (1975).
Ohtsuka, K., and Wakabayashi, M., Enzymologia, 38, 109–124 (1970).
Owens, J. W., Gammon, K., and Stahl, P., Archs Biochem. Biophys., 166, 258–272 (1975).
Distler, J., and Jourdian, G., J. biol. Chem., 248, 6772–6780 (1973).
Miller, G., Analyt. Chem., 31, 964 (1959).
Hickman, S., Shapiro, L., and Neufeld, E., Biochem. biophys. Res. Commun., 57, 55–61 (1973).
Regoeczi, E., Hatton, M. W. C., and Charlwood, P. A., Nature, 254, 699–701 (1975).
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STAHL, P., SCHLESINGER, P., RODMAN, J. et al. Recognition of lysosomal glycosidases in vivo inhibited by modified glycoproteins. Nature 264, 86–88 (1976). https://doi.org/10.1038/264086a0
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DOI: https://doi.org/10.1038/264086a0
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