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Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants

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Abstract

CELL separation techniques, the possibility of recognising different markers by immunofluorescence and functional tests, have subdivided cells once thought to be a homogeneous group into different subclasses. This development has been most prominent for the so-called mononuclear cells. A difficulty inherent in the classification of such subclasses (of, for example, mononuclear cells) is that it is often almost impossible to determine precisely what percentage of the cells belongs to a certain subclass, and whether the sum of the subclasses is equal to, lower than or even higher than 100% of the total of the cells analysed. A typical recent example was the identification of the “null” or K cell1,2. Such analysis is greatly facilitated if we analyse a cell suspension stained with two or more different fluorescent dyes visible at the same time. This is feasible.

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Authors and Affiliations

  1. Department of Immunohaematology, University Hospital Leiden, Leiden

    J. J. VAN ROOD & A. VAN LEEUWEN

  2. Department of Histochemistry and Cytochemistry, University of Leiden, Leiden, The Netherlands

    J. S. PLOEM

Authors
  1. J. J. VAN ROOD
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  2. A. VAN LEEUWEN
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  3. J. S. PLOEM
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VAN ROOD, J., VAN LEEUWEN, A. & PLOEM, J. Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants. Nature 262, 795–797 (1976). https://doi.org/10.1038/262795a0

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  • DOI: https://doi.org/10.1038/262795a0

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